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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

TGVEG_249180

Organism
Toxoplasma gondii
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei489 – 4891UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationImported, OxidoreductaseUniRule annotationImported, Transferase

Keywords - Biological processi

Nucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
Gene namesi
ORF Names:TGVEG_249180Imported
OrganismiToxoplasma gondiiImported
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma
ProteomesiUP000002226 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiToxoDB:TGME49_249180.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliB9PR34.
SMRiB9PR34. Positions 322-610.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

Phylogenomic databases

InParanoidiB9PR34.
OMAiICGSLDS.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 2 hits.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9PR34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKPVCLVVA MTPKRGIGIN NGLPWPHLTT DFKHFSRVTK TTPEEASRLN
60 70 80 90 100
GWLPRKFAKT GDSGLPSPSV GKRFNAVVMG RKTWESMPRK FRPLVDRLNI
110 120 130 140 150
VVSSSLKEED IAAEKPQAEG QQRVRVCASL PAALSLLEEE YKDSVDQIFV
160 170 180 190 200
VGGAGLYEAA LSLGVASHLY ITRVAREFPC DVFFPAFPGD DILSNKSTAA
210 220 230 240 250
QAAAPAESVF VPFCPELGRE KDNEATYRPI FISKTFSDNG VPYDFVVLEK
260 270 280 290 300
RRKTDDAATA EPSNAMSSLT STRETTPVHG LQAPSSAAAI APVLAWMDEE
310 320 330 340 350
DRKKREQKEL IRAVPHVHFR GHEEFQYLDL IADIINNGRT MDDRTGVGVI
360 370 380 390 400
SKFGCTMRYS LDQAFPLLTT KRVFWKGVLE ELLWFIRGDT NANHLSEKGV
410 420 430 440 450
KIWDKNVTRE FLDSRNLPHR EVGDIGPGYG FQWRHFGAAY KDMHTDYTGQ
460 470 480 490 500
GVDQLKNVIQ MLRTNPTDRR MLMTAWNPAA LDEMALPPCH LLCQFYVNDQ
510 520 530 540 550
KELSCIMYQR SCDVGLGVPF NIASYSLLTL MVAHVCNLKP KEFIHFMGNT
560 570 580 590 600
HVYTNHVEAL KEQLRREPRP FPIVNILNKE RIKEIDDFTA EDFEVVGYVP
610
HGRIQMEMAV
Length:610
Mass (Da):68,752
Last modified:March 24, 2009 - v1
Checksum:iCFAF564416D04A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAYL02000077 Genomic DNA. Translation: ESS34444.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAYL02000077 Genomic DNA. Translation: ESS34444.1.

3D structure databases

ProteinModelPortaliB9PR34.
SMRiB9PR34. Positions 322-610.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiB9PR34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

EuPathDBiToxoDB:TGME49_249180.

Phylogenomic databases

InParanoidiB9PR34.
OMAiICGSLDS.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 2 hits.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Annotation of Toxoplasma gondii VEG."
    Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 50861 / VEGImported.

Entry informationi

Entry nameiB9PR34_TOXGO
AccessioniPrimary (citable) accession number: B9PR34
Secondary accession number(s): B6KHC9, B9QD29
Entry historyi
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 27, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.