Bifunctional dihydrofolate reductase-thymidylate synthase

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B9PR34 (B9PR34_TOXGO) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 31. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase PIRNR PIRNR000389

Gene names

ORF Names:

TGGT1_100740 EMBL EEE23163.1, TGME49_049180 EMBL EEB00112.1, TGVEG_002710 EMBL EEE31708.1

Organism

Toxoplasma gondii [Reference proteome]

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Conoidasida › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism By similarity. PIRNR PIRNR000389

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. PIRNR PIRNR000389

Sequence similarities

In the C-terminal section; belongs to the thymidylate synthase family. PIRNR PIRNR000389

In the N-terminal section; belongs to the dihydrofolate reductase family. PIRNR PIRNR000389

Ontologies

Keywords
Biological process	Nucleotide biosynthesis PIRNR PIRNR000389 One-carbon metabolism PIRNR PIRNR000389
Molecular function	Methyltransferase PIRNR PIRNR000389 EMBL EEE23163.1 Oxidoreductase PIRNR PIRNR000389 EMBL EEE23163.1 Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro glycine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro thymidylate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Active site

489

By similarity PIRSR PIRSR000389-1

Sequences

Sequence

Length

Mass (Da)

Tools

B9PR34 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: CFAF564416D04A29
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FASTA

610

68,752

        10         20         30         40         50         60 
MQKPVCLVVA MTPKRGIGIN NGLPWPHLTT DFKHFSRVTK TTPEEASRLN GWLPRKFAKT 

        70         80         90        100        110        120 
GDSGLPSPSV GKRFNAVVMG RKTWESMPRK FRPLVDRLNI VVSSSLKEED IAAEKPQAEG 

       130        140        150        160        170        180 
QQRVRVCASL PAALSLLEEE YKDSVDQIFV VGGAGLYEAA LSLGVASHLY ITRVAREFPC 

       190        200        210        220        230        240 
DVFFPAFPGD DILSNKSTAA QAAAPAESVF VPFCPELGRE KDNEATYRPI FISKTFSDNG 

       250        260        270        280        290        300 
VPYDFVVLEK RRKTDDAATA EPSNAMSSLT STRETTPVHG LQAPSSAAAI APVLAWMDEE 

       310        320        330        340        350        360 
DRKKREQKEL IRAVPHVHFR GHEEFQYLDL IADIINNGRT MDDRTGVGVI SKFGCTMRYS 

       370        380        390        400        410        420 
LDQAFPLLTT KRVFWKGVLE ELLWFIRGDT NANHLSEKGV KIWDKNVTRE FLDSRNLPHR 

       430        440        450        460        470        480 
EVGDIGPGYG FQWRHFGAAY KDMHTDYTGQ GVDQLKNVIQ MLRTNPTDRR MLMTAWNPAA 

       490        500        510        520        530        540 
LDEMALPPCH LLCQFYVNDQ KELSCIMYQR SCDVGLGVPF NIASYSLLTL MVAHVCNLKP 

       550        560        570        580        590        600 
KEFIHFMGNT HVYTNHVEAL KEQLRREPRP FPIVNILNKE RIKEIDDFTA EDFEVVGYVP 

       610 
HGRIQMEMAV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Annotation of Toxoplasma gondii GT1." Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GT1.
[2]	"Annotation of Toxoplasma gondii VEG." Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 50861 / VEG.
[3]	Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: GT1 EMBL EEE23163.1 and VEG EMBL EEE31708.1.
[4]	"Annotation of Toxoplasma gondii ME49." Lorenzi H., Caler E., Galinsky K., Schobel S., Brunk B.P., Roos D.S., Paulsen I. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ME49 EMBL EEB00112.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS984732 Genomic DNA. Translation: EEB00112.1. EQ967481 Genomic DNA. Translation: EEE23163.1. EQ970684 Genomic DNA. Translation: EEE31708.1.
RefSeq	XP_002367252.1. XM_002367211.1.
UniGene	Tgo.2459.
3D structure databases
SMR	B9PR34. Positions 322-610.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	TGME49_049180; TGME49_049180; TGME49_049180.
GeneID	7899539.
KEGG	tgo:TGME49_049180.
Organism-specific databases
EuPathDB	ToxoDB:TGME49_249180.
Phylogenomic databases
KO	K13998.
OMA	CEAVHLT.
ProtClustDB	PTZ00164.
Enzyme and pathway databases
UniPathway	UPA00077; UER00158.
Family and domain databases
Gene3D	3.30.572.10. 1 hit. 3.40.430.10. 2 hits.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR024072. DHFR-like_dom. IPR012262. DHFR-TS. IPR017925. DHFR_CS. IPR001796. DHFR_dom. IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PIRSF	PIRSF000389. DHFR-TS. 1 hit.
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF53597. SSF53597. 1 hit. SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B9PR34_TOXGO

Accession

Primary (citable) accession number: B9PR34
Secondary accession number(s): B6KHC9, B9QD29

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	April 3, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information