Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Biotin carboxylase 2, chloroplastic

Gene

POPTR_0018s14250g

Organism
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.By similarity

Catalytic activityi

ATP + biotin-[carboxyl-carrier-protein] + HCO3- = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].By similarity
ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: malonyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA.
Proteins known to be involved in this subpathway in this organism are:
  1. Biotin carboxylase 2, chloroplastic (POPTR_0018s14250g), Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), Biotin carboxylase 1, chloroplastic (POPTRDRAFT_831870)
This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181ATPBy similarity1
Binding sitei265ATPBy similarity1
Binding sitei300ATPBy similarity1
Metal bindingi340Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi353Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi353Magnesium or manganese 2PROSITE-ProRule annotation1
Metal bindingi355Magnesium or manganese 2PROSITE-ProRule annotation1
Active sitei357By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi213 – 274ATPPROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Biotin, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00655; UER00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin carboxylase 2, chloroplastic (EC:6.3.4.14)
Alternative name(s):
Acetyl-CoA carboxylase subunit A 2 (EC:6.4.1.2)
Short name:
ACC
Gene namesi
ORF Names:POPTR_0018s14250g
OrganismiPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifieri3694 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus
Proteomesi
  • UP000006729 Componentsi: Linkage group LGXVIII, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 71ChloroplastSequence analysisAdd BLAST71
ChainiPRO_000039177472 – 526Biotin carboxylase 2, chloroplasticAdd BLAST455

Proteomic databases

PRIDEiB9N843.

Interactioni

Subunit structurei

Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein, biotin carboxylase and two subunits each of ACCase subunit alpha and ACCase plastid-coded subunit beta (accD).Curated

Protein-protein interaction databases

STRINGi3694.POPTR_0018s14250.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 382ATP-graspPROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IU5C. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000008988.
InParanoidiB9N843.
KOiK01961.
OMAiKIESYHP.
OrthoDBiEOG093607T8.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR004549. Acetyl_CoA_COase_biotin_COase.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00514. accC. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9N843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATLPVCKS VTSTPGLFMK RNSGIRNSQC SFMVGTKVNF PRQRTQATQA
60 70 80 90 100
NHCAKKNGGA LGVTCRAEKI LVANRGEIAV RVIRTAHELG IPCVAVYSTI
110 120 130 140 150
DKDALHVKLA DESVCIGEAP SNQSYLVIQN VLSAAISRGC TMLHPGYGFL
160 170 180 190 200
AENAVFVEMC REHGINFIGP NPDSIRVMGD KSTARETMKK ANVPTVPGSD
210 220 230 240 250
GLLQSTEEAV KLASEIGYPV MIKATAGGGG RGMRLAKEPD EFVKLLQQAK
260 270 280 290 300
SEAAAAFGND GVYLEKYVQN PRHIEFQVLA DKFGNVVHFG ERDCSIQRRN
310 320 330 340 350
QKLLEEAPSP ALTPELRKAM GDAAVAAAAS IGYIGVGTVE FLLDERGSFY
360 370 380 390 400
FMEMNTRIQV EHPVTEMISS VDLIEEQIRV AMGEKIQYKQ EDIVLRGHSI
410 420 430 440 450
ECRINAEDAF KGFRPGPGRI TAYLPSGGPF VRMDSHVYPD YVVPPSYDSL
460 470 480 490 500
LGKLIVWAPT REKAIERMKR ALDDTIITGV PTTIDYHKLI LDIEDFKNGN
510 520
VDTAFIPKHE QELAAPQQII LANSAS
Length:526
Mass (Da):57,497
Last modified:February 19, 2014 - v2
Checksum:iCAC6A56109E42892
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000354 Genomic DNA. Translation: ERP49997.1.
RefSeqiXP_006372200.1. XM_006372138.1.

Genome annotation databases

EnsemblPlantsiPOPTR_0018s14250.1; POPTR_0018s14250.1; POPTR_0018s14250.
GeneIDi18107844.
GrameneiPOPTR_0018s14250.1; POPTR_0018s14250.1; POPTR_0018s14250.
KEGGipop:POPTR_0018s14250g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000354 Genomic DNA. Translation: ERP49997.1.
RefSeqiXP_006372200.1. XM_006372138.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3694.POPTR_0018s14250.1.

Proteomic databases

PRIDEiB9N843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiPOPTR_0018s14250.1; POPTR_0018s14250.1; POPTR_0018s14250.
GeneIDi18107844.
GrameneiPOPTR_0018s14250.1; POPTR_0018s14250.1; POPTR_0018s14250.
KEGGipop:POPTR_0018s14250g.

Phylogenomic databases

eggNOGiENOG410IU5C. Eukaryota.
COG0439. LUCA.
HOGENOMiHOG000008988.
InParanoidiB9N843.
KOiK01961.
OMAiKIESYHP.
OrthoDBiEOG093607T8.

Enzyme and pathway databases

UniPathwayiUPA00655; UER00711.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR004549. Acetyl_CoA_COase_biotin_COase.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00514. accC. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACCC2_POPTR
AccessioniPrimary (citable) accession number: B9N843
Secondary accession number(s): U5FKU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: February 19, 2014
Last modified: September 7, 2016
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.