Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
Binding sitei604 – 6041ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCBES521460:GH8H-1368-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Athe_1349
OrganismiCaldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Taxonomic identifieri521460 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
ProteomesiUP000007723 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_1000189120Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi521460.Athe_1349.

Structurei

3D structure databases

ProteinModelPortaliB9MRZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi601 – 6055"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9MRZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWSQTLNLP KTDFPMRANL AQREPQFLKF WQENDIFKKM LEKNKNNKKF
60 70 80 90 100
ILHDGPPYAN GDIHLGHALN KVLKDIVNKY KSLQGYYTPY IPGWDTHGLP
110 120 130 140 150
IEQQVIKKLE VNRHEVDPVE FRKKCKEFAL SYIDIQRQQF KRLGVFGEWE
160 170 180 190 200
NPYMTLDPKF EARQIRVFGE MAKKGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YQEDRTYSIY VKFEVIDDKG LFSNLPIGDK KVYIVIWTTT TWTLPGNLAI
260 270 280 290 300
ALNADFDYSL IDIGNEILVV ASELVERVMK TNKIEQYKEI ARFKGKDLEY
310 320 330 340 350
VKCKHPFLDR TSLVILGEHV TLEAGTGCVH TAPGHGEEDF EVCQRYNIHV
360 370 380 390 400
IVPVDNKGYL TKEAGKFAGL FYEDSNKEIA KELEASGHLL GVEKITHQYP
410 420 430 440 450
HCWRCKNPVI FRATEQWFAS VKGFRDQALK AVDDVKWVPE WGRDRIYNMI
460 470 480 490 500
VDRQDWCISR QRIWGVPIPI FYCKNCRKEL ITDETIDYIA KIFEKEGSDA
510 520 530 540 550
WFSKDVKELL PEGVKCPVCG CSEFEKETDI MDVWFDSGSS HAYVLESRED
560 570 580 590 600
LEWPCDMYLE GNDQYRGWFQ SSLLTAVATK GRAPYRIVLT HGFVVDGEGK
610 620 630 640 650
KMSKSEGNVI SPFDIINEFG ADILRLWCVS ADYTTDMRIS KDIIKQLTEI
660 670 680 690 700
YRKIRNTARF LLGNLYDFNP KTDKVGYENL KEIDKWALQR LYKLIEKVTK
710 720 730 740 750
AYEEYDYNQV YHLVHNFCVI DMSNLYLDIN KDRLYASKSE SLDRRSAQTV
760 770 780 790 800
MYEILIALTI LIAPILSFTA EEIWQNIIFK EEDAESVFLT RWPSINEDIL
810 820 830 840 850
RDEALREKWD KIIEIKDIVS KQLEIARNEK LIGSSLDSKV KIFAKCDIKR
860 870 880 890 900
FIEENKDIIQ EVLIVSQLDV EESDSDQIKV EVYKADGSKC ERCWKFDTMV
910 920
GKNEESLNVC PRCYEVVKSK
Length:920
Mass (Da):107,205
Last modified:March 24, 2009 - v1
Checksum:i64EB550849406D86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001393 Genomic DNA. Translation: ACM60449.1.
RefSeqiWP_015907819.1. NC_012034.1.
YP_002573222.1. NC_012034.1.

Genome annotation databases

EnsemblBacteriaiACM60449; ACM60449; Athe_1349.
KEGGiate:Athe_1349.
PATRICi20899814. VBIAnaThe135187_1420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001393 Genomic DNA. Translation: ACM60449.1.
RefSeqiWP_015907819.1. NC_012034.1.
YP_002573222.1. NC_012034.1.

3D structure databases

ProteinModelPortaliB9MRZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi521460.Athe_1349.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM60449; ACM60449; Athe_1349.
KEGGiate:Athe_1349.
PATRICi20899814. VBIAnaThe135187_1420.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCBES521460:GH8H-1368-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kataeva I., Adams M.W.W.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1888 / DSM 6725 / Z-1320.

Entry informationi

Entry nameiSYI_CALBD
AccessioniPrimary (citable) accession number: B9MRZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 29, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.