B9MQB5 (B9MQB5_CALBD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase HAMAP-Rule MF_00140
EC=6.1.1.2 HAMAP-Rule MF_00140

Alternative name(s):
Tryptophanyl-tRNA synthetase HAMAP-Rule MF_00140

Gene names

Name:	trpS HAMAP-Rule MF_00140
Ordered Locus Names:	Athe_0792 EMBL ACM59907.1

Organism

Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum) [Complete proteome] [HAMAP] EMBL ACM59907.1

Taxonomic identifier

521460 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor › Caldicellulosiruptor bescii

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). SAAS SAAS024109 HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00140
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00140.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. RuleBase RU003489 HAMAP-Rule MF_00140

Ontologies

Keywords
Biological process	Protein biosynthesis RuleBase RU003489 SAAS SAAS024109 HAMAP-Rule MF_00140
Cellular component	Cytoplasm HAMAP-Rule MF_00140
Ligand	ATP-binding SAAS SAAS024109 RuleBase RU003489 HAMAP-Rule MF_00140 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase RuleBase RU003489 SAAS SAAS024109 HAMAP-Rule MF_00140 EMBL ACM59907.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	10 – 18	9	"HIGH" region By similarity HAMAP-Rule MF_00140
	Motif	193 – 197	5	"KMSKS" region By similarity HAMAP-Rule MF_00140
Sites
	Binding site	196	1	ATP By similarity HAMAP-Rule MF_00140

Sequences

	Sequence		Length	Mass (Da)
	B9MQB5 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 9E1E03ADC42467AC	FASTA	325	37,305
	10 20 30 40 50 60 MRRVLSGTRP TGILHLGNYF GAIESWVKLQ DEYECFFFVA DWHALTTGYE DTSNLREYTK 70 80 90 100 110 120 QVVIDLLACG LDPKKCTIFV QSHVPQHAEL HLLFSMIMPL SWLYRCPTYK DQMRELKERN 130 140 150 160 170 180 IATYGFLGYP CLQAADILIY KAELVPVGED QLPHLELTRE IARRFNFLYG QTFPEPQAIL 190 200 210 220 230 240 NTVKVLVGTD GRKMSKSYGN TIALSEDLES VRKKVMNMVT DPARIRKNDP GHPEVCTVFA 250 260 270 280 290 300 YHQIFSPETV SETEEDCRQG KIGCVECKKR LFENISRFLE PIQSKRRELE NDLDYVLGVI 310 320 NEGARKAREI ASRTLQEAKD RAGLL

References

[1]

"Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kataeva I., Adams M.W.W. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1888 / DSM 6725 / Z-1320.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001393 Genomic DNA. Translation: ACM59907.1.
RefSeq	YP_002572680.1. NC_012034.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	521460.Athe_0792.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM59907; ACM59907; Athe_0792.
GeneID	7407979.
KEGG	ate:Athe_0792.
PATRIC	20898655. VBIAnaThe135187_0844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059941.
KO	K01867.
OMA	THYRETE.
ProtClustDB	PRK00927.
Enzyme and pathway databases
BioCyc	CBES521460:GH8H-821-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B9MQB5_CALBD

Accession

Primary (citable) accession number: B9MQB5

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)