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B9MQ24

- LIPA_CALBD

UniProt

B9MQ24 - LIPA_CALBD

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Protein
Lipoyl synthase
Gene
lipA, Athe_0701
Organism
Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi62 – 621Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBES521460:GH8H-712-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Athe_0701
OrganismiCaldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Taxonomic identifieri521460 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
ProteomesiUP000007723: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Lipoyl synthaseUniRule annotation
PRO_1000124617Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi521460.Athe_0701.

Structurei

3D structure databases

ProteinModelPortaliB9MQ24.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9MQ24-1 [UniParc]FASTAAdd to Basket

« Hide

MSYLKKPDWL KIRVKADQKI DDVIEILKMF SLHTVCEEAQ CPNIYECFSK    50
KTATFLIMGD VCTRNCTFCD VKKGKPVKLN SDEPKMVANA VGALGLKYVV 100
ITSVTRDDLP DGGASHFAEC IRSIKGKRPY TKIEVLIPDF KGSFESVSKV 150
VEASPDVVAH NIETIERLYP CVRPLASYKR SLDVLRMVKE IDKNIFTKSG 200
IMVGLGETKD EVKKALEDLR NAECDFVTIG QYLSPSKNHH PVVEFVHPDV 250
FEEYKEFAIS IGFKFVMSGP LVRSSYMAEN TKDIIENVRK I 291
Length:291
Mass (Da):32,828
Last modified:March 24, 2009 - v1
Checksum:iD4E26A79F036152F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001393 Genomic DNA. Translation: ACM59816.1.
RefSeqiWP_015907258.1. NC_012034.1.
YP_002572589.1. NC_012034.1.

Genome annotation databases

EnsemblBacteriaiACM59816; ACM59816; Athe_0701.
GeneIDi7407125.
KEGGiate:Athe_0701.
PATRICi20898455. VBIAnaThe135187_0750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001393 Genomic DNA. Translation: ACM59816.1 .
RefSeqi WP_015907258.1. NC_012034.1.
YP_002572589.1. NC_012034.1.

3D structure databases

ProteinModelPortali B9MQ24.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521460.Athe_0701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM59816 ; ACM59816 ; Athe_0701 .
GeneIDi 7407125.
KEGGi ate:Athe_0701.
PATRICi 20898455. VBIAnaThe135187_0750.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CBES521460:GH8H-712-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kataeva I., Adams M.W.W.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1888 / DSM 6725 / Z-1320.

Entry informationi

Entry nameiLIPA_CALBD
AccessioniPrimary (citable) accession number: B9MQ24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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