B9MPC3 (B9MPC3_ANATD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	Athe_0558

Organism

Anaerocellum thermophilum (strain DSM 6725 / Z-1320) [Complete proteome] [HAMAP]

Taxonomic identifier

521460 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	318	1	Nucleophile By similarity HAMAP MF_00685
	Active site	365	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B9MPC3 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 4C7F300C10C8B603	FASTA	650	76,859
	10 20 30 40 50 60 MIKKVKSTIY LSDIKKFESG EHFESYKFLG SKVVNYRGKV GTVFCVWAPN AKSVSVVGNF 70 80 90 100 110 120 NNWRGENHKM MRVYGSGFWW LFVEGIGEGE LYKYEIIGAD GKRVLKADPY AIYSEKRPNT 130 140 150 160 170 180 ASIVKNIPDY EWHDQEWMEK RKTTPPYDKP INIYEVHLAS WKMKKDGSIE KAGEFYNYRE 190 200 210 220 230 240 LAHMLVDYIK EMNYNYIELL PVLEHPLDMS WGYQPTGYFS LTSRYGSIED FMYFVDYMHQ 250 260 270 280 290 300 NGIGVIVDWV PAHFCKDEHG LYRFDGTFLY EYEDELLREN YTWGTATFDF AKPQVQSFLI 310 320 330 340 350 360 SSAMFWFDVY HIDGIRVDAV SHIIYMNNNQ KNRYGGHENI EGIEFIKKLN KAIFSKYPNV 370 380 390 400 410 420 LMIAEESTAF PLVTYPTYDG GLGFNYKWNM GWMNDTLKYM QKHPDERKQH HNLLTFSIMY 430 440 450 460 470 480 AFSENFILPF SHDEVVHGKK SLLDKMPGDY NQKFANLRLL YGYMYTHPGK KLLFMGGEFG 490 500 510 520 530 540 QFIEWRFYAS LDWLLLDYPM HRMLQHYVKS LNRFYLENKA LWELDHKMNG FRWIDVHNWE 550 560 570 580 590 600 QSVISYLRIS KEPDDYLVVI CNFSLASYEN YKIGVPKKGI YLEVFNSDKA EFGGNNIVNT 610 620 630 640 650 EKLKTIDEVW HGYNQCIEFR LPALSCLIFK PIEFFNAQEE KNQNDNNIQI

References

[1]

"Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kataeva I., Adams M.W.W. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001393 Genomic DNA. Translation: ACM59684.1.
RefSeq	YP_002572457.1. NC_012034.1.
3D structure databases
ProteinModelPortal	B9MPC3.
ModBase	Search...
Protein-protein interaction databases
STRING	B9MPC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7408684.
GenomeReviews	Gene locus Athe_0558 in contig CP001393_GR.
KEGG	ate:Athe_0558.
PATRIC	20898136. VBIAnaThe135187_0591.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RVYHQNG.
ProtClustDB	CLSK2472773.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B9MPC3_ANATD

Accession

Primary (citable) accession number: B9MPC3

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)