B9MJX8 (ISPE_ANATD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Athe_1538

Organism

Anaerocellum thermophilum (strain DSM 6725 / Z-1320) [Complete proteome] [HAMAP]

Taxonomic identifier

521460 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000117875
Regions
	Nucleotide binding	92 – 102	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	134	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9MJX8 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 9A3BCC67E2F59F15	FASTA	286	31,279
	10 20 30 40 50 60 MKLKAYAKIN LALDVLSKRE DGYHEIRTIM QTVDLYDIIN IEKIEEDNII VTTSSENIPT 70 80 90 100 110 120 DNKNHAYIAA SLLKERFGVK QGVRIHIEKN IPVSAGLAGG STDAAAVLKG LNEIFELNLS 130 140 150 160 170 180 EQQLMEIGRE IGADVPFCLV GGTALCEGIG EKVIKLKSAP QMNILIAKPE VYVSTQAVYE 190 200 210 220 230 240 ALDLSKIKKR PNIEAMISAI EEGNVKEIAK NLCNVLEVVT VNQYPVINRV KDIMRNNNAL 250 260 270 280 GTVMTGSGPA VFGIFGNKYN ALKAAERLKV FIKEIILTTT CEGSGF

References

[1]

"Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kataeva I., Adams M.W.W. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6725 / Z-1320.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001393 Genomic DNA. Translation: ACM60636.1.
RefSeq	YP_002573409.1. NC_012034.1.
3D structure databases
ProteinModelPortal	B9MJX8.
ModBase	Search...
Protein-protein interaction databases
STRING	B9MJX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7409045.
GenomeReviews	Gene locus Athe_1538 in contig CP001393_GR.
KEGG	ate:Athe_1538.
PATRIC	20900208. VBIAnaThe135187_1609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VSTAEVY.
ProtClustDB	PRK00128.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ANATD

Accession

Primary (citable) accession number: B9MJX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program