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B9MJH4

- BIOB_ACIET

UniProt

B9MJH4 - BIOB_ACIET

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Protein
Biotin synthase
Gene
bioB, Dtpsy_1855
Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi122 – 1221Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi213 – 2131Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi285 – 2851Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAEBR535289:GHOO-1886-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Dtpsy_1855
OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Taxonomic identifieri535289 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
ProteomesiUP000000450: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Biotin synthaseUniRule annotation
PRO_0000381354Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi535289.Dtpsy_1855.

Structurei

3D structure databases

ProteinModelPortaliB9MJH4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9MJH4-1 [UniParc]FASTAAdd to Basket

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MTSATTAPHT TQTLQFHPRV AESVATQRGE GWSIQAVQEL LDLPFMELLW    50
RAQATHRAHW PQGDIELATL LSVKTGGCPE NCGYCPQSAE FDTGVKAEKL 100
MSVQEVTQAA QAAKDAGATR FCMGAAWRAP KDRDIEKMSE LITAVKDLGL 150
QTCATLGMLQ SHQALALKDA GLDYYNHNLD TAPEYYSDVV STRQYQDRLD 200
TLRHVRDAGI NVCCGGIVGM GEAPVHRAGL IAQLANLNPY PESVPINSLV 250
RVAGTPLANS EPVDPLDFVR VIAVARITMP KARVRLSAGR QQLGDAVQAL 300
CFLAGANSIF YGDKLLVTSN PDVEADTQLL AKLGLKGTPN QTTTETACGA 350
Length:350
Mass (Da):37,658
Last modified:March 24, 2009 - v1
Checksum:i344F8A60A311D866
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001392 Genomic DNA. Translation: ACM33312.1.
RefSeqiYP_002553312.1. NC_011992.1.

Genome annotation databases

EnsemblBacteriaiACM33312; ACM33312; Dtpsy_1855.
GeneIDi7382216.
KEGGidia:Dtpsy_1855.
PATRICi21780821. VBIDiaSp55748_1897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001392 Genomic DNA. Translation: ACM33312.1 .
RefSeqi YP_002553312.1. NC_011992.1.

3D structure databases

ProteinModelPortali B9MJH4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 535289.Dtpsy_1855.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM33312 ; ACM33312 ; Dtpsy_1855 .
GeneIDi 7382216.
KEGGi dia:Dtpsy_1855.
PATRICi 21780821. VBIDiaSp55748_1897.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AEBR535289:GHOO-1886-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TPSY.

Entry informationi

Entry nameiBIOB_ACIET
AccessioniPrimary (citable) accession number: B9MJH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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