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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 748ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAEBR535289:GHOO-1580-MONOMER.
UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:Dtpsy_1554
OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Taxonomic identifieri535289 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
Proteomesi
  • UP000000450 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Tetraacyldisaccharide 4'-kinasePRO_1000134737Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi535289.Dtpsy_1554.

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004953.
KOiK00912.
OMAiKLWRTHP.
OrthoDBiPOG091H000L.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

B9MI83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGRPSPL AERLRGAWQH RGALALALWP LSLLYGVLTA LRRALYRTGL
60 70 80 90 100
LRSERLPVPV IVVGNVIAGG AGKTPVTLAV VRHLQARGWR PGVISRGYGR
110 120 130 140 150
ATVDCREVLP ASSAAEVGDE PLLIARASGA PVFVARRRAQ AGRALLAAHP
160 170 180 190 200
ATNILVCDDG LQHLALARDL EVCVFNDEGA GNGWMLPAGP LREPWPRAVD
210 220 230 240 250
LVLHAGSSPG GGAPQFALSR ELATHAVNAS GHQLPLEQLQ GEPSHALAAI
260 270 280 290 300
ARPHDFFAML HARGIQPESE EALPDHYDFS SWKRPPDKRL RLICTEKDAV
310 320 330
KLWPAHPDAL AVPLALRIPP AFFDALDARL ASLSSSGH
Length:338
Mass (Da):36,097
Last modified:March 24, 2009 - v1
Checksum:i733279922F8C92A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM33015.1.
RefSeqiWP_015913123.1. NC_011992.1.

Genome annotation databases

EnsemblBacteriaiACM33015; ACM33015; Dtpsy_1554.
KEGGidia:Dtpsy_1554.
PATRICi21780195. VBIDiaSp55748_1589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM33015.1.
RefSeqiWP_015913123.1. NC_011992.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi535289.Dtpsy_1554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM33015; ACM33015; Dtpsy_1554.
KEGGidia:Dtpsy_1554.
PATRICi21780195. VBIDiaSp55748_1589.

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004953.
KOiK00912.
OMAiKLWRTHP.
OrthoDBiPOG091H000L.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.
BioCyciAEBR535289:GHOO-1580-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXK_ACIET
AccessioniPrimary (citable) accession number: B9MI83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.