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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei661 – 6611ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAEBR535289:GHOO-3331-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Dtpsy_3266
OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Taxonomic identifieri535289 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
ProteomesiUP000000450: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Leucine--tRNA ligasePRO_1000199198Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi535289.Dtpsy_3266.

Structurei

3D structure databases

ProteinModelPortaliB9MH67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi658 – 6625"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9MH67-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQDKYNHTEV ERAAHAHWNA NDAYRVTEDQ AKPKFYACSM LPYPSGKLHM
60 70 80 90 100
GHVRNYTIND MLTRSLRMKG HNVLMPMGWD AFGLPAENAA LKNGVPPAQW
110 120 130 140 150
TYDNIAYMKK QMQAMGLAID WSREVATCDP DYYKWNQWLF LKMLDKGIAY
160 170 180 190 200
RKTQVVNWDP VDQTVLANEQ VIDGRGWRTG ALVEKREIPG YYLKITDYAQ
210 220 230 240 250
ELLDHVQIGN EKATLTGWPD KVRLMQENWI GKSAGVRFAF PHDIRNAAGE
260 270 280 290 300
RIQDGKLYVF TTRADTIMGV TFCAVAPEHP LAQHAAASNA PLAAFIEECK
310 320 330 340 350
KGGTTEAELA LKEKEGMPTG LFVTHPLTGE QVEVWVGNYV LMSYGDGAVM
360 370 380 390 400
GVPAHDERDF AFALKYQLPI KQVVLVDGET FDFHQWQDWY GDKERGVTIN
410 420 430 440 450
SDNFSGLSYQ DAVAAVAHAL AEKGLGELKT TWRLRDWGIS RQRYWGTPIP
460 470 480 490 500
IIHCESCGAV PVPEKDLPVV LPQDLVPDGS GNPLAKCEAF LKVDCPCCGK
510 520 530 540 550
PARRETDTMD TFVDSSWYFM RYCDPKNRDA MVAGGTDYWM RDQKAATGGS
560 570 580 590 600
GMDQYIGGIE HAILHLLYAR FWTKVMRDLG LVKVDEPFTK LLTQGMVLNH
610 620 630 640 650
IYSRRTAKGA KDYFWPHDVE HVYDEAGKIV GAKLKNPAES GDGLLPVGTP
660 670 680 690 700
IDYEGVGTMS KSKNNGVDPQ QLIEKYGADT ARLYTMFTAP PELTLEWNDA
710 720 730 740 750
AVEGSYRFLR RVWNFGVKLS AIDKDAALAS VAGAASLKDV QFGKEAKALR
760 770 780 790 800
LEIHTVLKQV DYDYQRMQYN TVVSGAMKMI NALEDFKATD SAGAQVALIE
810 820 830 840 850
GFGILLRVLY PATPHIAHVL WDELGYAGTL GDLLDAAWPQ VAPDALVQDE
860 870 880 890 900
LELMLQVNGK LRGAIRVAAS ADKAAIEQAA LASEDFQKFA EGKAPKKVII
910
VPGRLVNVVV
Length:910
Mass (Da):101,266
Last modified:March 24, 2009 - v1
Checksum:iA75017841E67CA7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM34695.1.
RefSeqiYP_002554695.1. NC_011992.1.

Genome annotation databases

EnsemblBacteriaiACM34695; ACM34695; Dtpsy_3266.
GeneIDi7385165.
KEGGidia:Dtpsy_3266.
PATRICi21783707. VBIDiaSp55748_3308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM34695.1.
RefSeqiYP_002554695.1. NC_011992.1.

3D structure databases

ProteinModelPortaliB9MH67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi535289.Dtpsy_3266.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM34695; ACM34695; Dtpsy_3266.
GeneIDi7385165.
KEGGidia:Dtpsy_3266.
PATRICi21783707. VBIDiaSp55748_3308.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAEBR535289:GHOO-3331-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TPSY.

Entry informationi

Entry nameiSYL_ACIET
AccessioniPrimary (citable) accession number: B9MH67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.