B9MH63 (PROA_ACIET) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Dtpsy_3262

Organism

Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY)) [Complete proteome] [HAMAP]

Taxonomic identifier

535289 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000193598

Sequences

	Sequence		Length	Mass (Da)
	B9MH63 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 9BDD1FAE2DB5FCEB	FASTA	426	45,274
	10 20 30 40 50 60 MNALNIAEYT HTLGLQAKTA SAQMARAPAA IKNKALLALA RLLRQNVDAL QGDNARDLER 70 80 90 100 110 120 ARAAGLAEPM VDRLKLTPKV LETCAQGCEQ LAAMPDIIGE IQGMKQQPSG IRVGQMRVPI 130 140 150 160 170 180 GVFGMIYESR PNVTIEAASL SIKSGNACIL RGGSEAIDSN KALARLVAQA LAEAGLPEHG 190 200 210 220 230 240 VQLVQTTDRA AVGQLIAMPQ YVDVIIPRGG KGLIERISAE AKVPVIKHLD GNCHTYVDDP 250 260 270 280 290 300 CDIVMAVQVA DNAKTQKYSP CNASEGLLVA RGVAAQFLPQ IGAVYAAKGV EMRGCPEALA 310 320 330 340 350 360 ILRAVPGVQL AEATEADWSE EYLAPIISVK VVAGVDEAIA HINRYGSHHT DAILTRDHMH 370 380 390 400 410 420 AQQFLRDVDS ASVMVNASTR FADGFEYGLG AEIGISTDKF HARGPVGIEG LTSLKWVVLG EGEVRT

References

[1]

"Complete sequence of Diaphorobacter sp. TPSY." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Coates J.D. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TPSY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001392 Genomic DNA. Translation: ACM34691.1.
RefSeq	YP_002554691.1. NC_011992.1.
3D structure databases
ProteinModelPortal	B9MH63.
ModBase	Search...
Protein-protein interaction databases
STRING	B9MH63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7383821.
GenomeReviews	Gene locus Dtpsy_3262 in contig CP001392_GR.
KEGG	dia:Dtpsy_3262.
PATRIC	21783699. VBIDiaSp55748_3304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YGICGAM.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIET

Accession

Primary (citable) accession number: B9MH63

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program