B9MG14 (CLPP_ACIET) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:	clpP
Ordered Locus Names:	Dtpsy_1128

Organism

Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY)) [Complete proteome] [HAMAP]

Taxonomic identifier

535289 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	ATP-dependent Clp protease proteolytic subunit HAMAP MF_00444		PRO_1000135153
Sites
	Active site	106	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9MG14 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 0A1A659CA77C025D	FASTA	202	22,257
	10 20 30 40 50 60 MSALETQALG MIPMVIEQSG RGERSYDIYS RLLKERVIFL VGEVNDQTAN LVVAQLLFLE 70 80 90 100 110 120 SENPDKDISF YINSPGGSVT AGMAIYDTMQ FIKPDVSTLC CGFAASMGAF LLAAGAKGKR 130 140 150 160 170 180 YSLPNSKIMI HQVLGGARGQ ATDIEIHARD ILRTKDQMNR ILAERTGQPI EKVKADTERD 190 200 YFMTADEAKD YGIVDQVIAK RP

References

[1]

"Complete sequence of Diaphorobacter sp. TPSY." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Coates J.D. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TPSY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001392 Genomic DNA. Translation: ACM32605.1.
RefSeq	YP_002552605.1. NC_011992.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B9MG14.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7384557.
GenomeReviews	Gene locus Dtpsy_1128 in contig CP001392_GR.
KEGG	dia:Dtpsy_1128.
PATRIC	21779318. VBIDiaSp55748_1160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KHDINKI.
ProtClustDB	PRK00277.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. False negative. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_ACIET

Accession

Primary (citable) accession number: B9MG14

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program