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B9MFT0

- DAPF_ACIET

UniProt

B9MFT0 - DAPF_ACIET

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei46 – 461SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei170 – 1701SubstrateUniRule annotation
    Sitei172 – 1721Important for catalytic activityUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei221 – 2211Important for catalytic activityUniRule annotation
    Active sitei230 – 2301Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciAEBR535289:GHOO-3045-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Dtpsy_2998
    OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
    Taxonomic identifieri535289 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
    ProteomesiUP000000450: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Diaminopimelate epimerasePRO_1000124412Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 230

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi535289.Dtpsy_2998.

    Structurei

    3D structure databases

    ProteinModelPortaliB9MFT0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni221 – 2222Substrate bindingUniRule annotation
    Regioni231 – 2322Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKDITVMV.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9MFT0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHIRFTKMQG AGNDFVVLDE TQGRLGLTPA QYRFLADRHF GVGADQILTV    50
    RPSPGAGIDF EYVIHNADGG QVEQCGNGAR CFARYVRDKG LTAQDTIRVQ 100
    TMAGVIAPHL TADGRVTVDM GRPELEPARV PFDTTGLAPV SQGSGQKWPL 150
    ALDGQASEAT LLVAVVSMGN PHAVQLVDDV DTAPVAQTGP LIESHPRFPQ 200
    RVNAGYLQIV NRGEVRLRVY ERGAGETLAC GTGACAAVVA GIRLGLLDAR 250
    VQVHTRGGLL TIDWAGGLQD PVFMTGPATT VFEGEIDIPD AL 292
    Length:292
    Mass (Da):31,002
    Last modified:March 24, 2009 - v1
    Checksum:iE8779E1A34325A82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001392 Genomic DNA. Translation: ACM34431.1.
    RefSeqiYP_002554431.1. NC_011992.1.

    Genome annotation databases

    EnsemblBacteriaiACM34431; ACM34431; Dtpsy_2998.
    GeneIDi7385443.
    KEGGidia:Dtpsy_2998.
    PATRICi21783151. VBIDiaSp55748_3045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001392 Genomic DNA. Translation: ACM34431.1 .
    RefSeqi YP_002554431.1. NC_011992.1.

    3D structure databases

    ProteinModelPortali B9MFT0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 535289.Dtpsy_2998.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM34431 ; ACM34431 ; Dtpsy_2998 .
    GeneIDi 7385443.
    KEGGi dia:Dtpsy_2998.
    PATRICi 21783151. VBIDiaSp55748_3045.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KDITVMV.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci AEBR535289:GHOO-3045-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TPSY.

    Entry informationi

    Entry nameiDAPF_ACIET
    AccessioniPrimary (citable) accession number: B9MFT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3