B9MFP8 (AROE_ACIET) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Dtpsy_2966

Organism

Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY)) [Complete proteome] [HAMAP]

Taxonomic identifier

535289 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000124882
Regions
	Nucleotide binding	130 – 134	5	NADP By similarity
Sites
	Active site	69	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	B9MFP8 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: F0C0C0973ABF4CB3	FASTA	282	29,172
	10 20 30 40 50 60 MTAAADLYCV MGNPIAHSRS PWIHARFAQL TDQSLHYERR LVPLDGFAQA LQSFAAEGGR 70 80 90 100 110 120 GCNITVPFKL EAAQLATTRS ERVQLAGAAN TLVFDGGSIH ADNTDGLGLV ADITQGAGVP 130 140 150 160 170 180 LAGRDVLLVG AGGAAAGVLG PLLRQHPRRI AVVNRTPARA QALVDSHAAL AALQKTELLA 190 200 210 220 230 240 LDMQAPGADF DVIINATASS LEGGPVPVPA SVLRPGSLAY DMMYGPAAQP FLDWASAHGA 250 260 270 280 TARDGLGMLV EQAAEAFLLW RGVRPPSAPV LQELRAAIAA GA

References

[1]

"Complete sequence of Diaphorobacter sp. TPSY." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Coates J.D. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TPSY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001392 Genomic DNA. Translation: ACM34399.1.
RefSeq	YP_002554399.1. NC_011992.1.
3D structure databases
ProteinModelPortal	B9MFP8.
ModBase	Search...
Protein-protein interaction databases
STRING	B9MFP8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7385411.
GenomeReviews	Gene locus Dtpsy_2966 in contig CP001392_GR.
KEGG	dia:Dtpsy_2966.
PATRIC	21783085. VBIDiaSp55748_3012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVFGNPI.
ProtClustDB	PRK00258.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_ACIET

Accession

Primary (citable) accession number: B9MFP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program