Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD kinase

Gene

nadK

Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80Proton acceptorUniRule annotation1
Binding sitei182NADUniRule annotation1
Binding sitei184NADUniRule annotation1
Binding sitei219NAD; via carbonyl oxygenUniRule annotation1
Binding sitei253NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi80 – 81NADUniRule annotation2
Nucleotide bindingi154 – 155NADUniRule annotation2
Nucleotide bindingi195 – 200NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Dtpsy_0833
OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Taxonomic identifieri535289 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
Proteomesi
  • UP000000450 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001335691 – 298NAD kinaseAdd BLAST298

Interactioni

Protein-protein interaction databases

STRINGi535289.Dtpsy_0833.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLAINCGR.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B9ME57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPIFRRVAV IGKYPGPGAV SASDSARQII ESIAQFVTQQ DCELTLEAET
60 70 80 90 100
AAHTGLTQYH TLDVEGIGRQ CDLCLVVGGD GTMLGVGRRL AGYGTPLVGI
110 120 130 140 150
NQGRLGFITD IPLEGYQDAL TPILHGDYEE DVRPLMQACV MRGGECVFEA
160 170 180 190 200
LALNDVVVNR GSTSGMVELR VEVDGVFVSN QRADGLIVAS PTGSTAYALS
210 220 230 240 250
AGGPMLHPSI PGWVLVPIAP HTLSNRPIVL SDATEVAIEV AGGRDISANF
260 270 280 290
DMQSLASLQH GDRILVRRSA HRVCFLHPRG WSYFATLRKK LGWYEGGS
Length:298
Mass (Da):31,885
Last modified:March 24, 2009 - v1
Checksum:i260FFA0A32F1C53C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM32311.1.
RefSeqiWP_012655803.1. NC_011992.1.

Genome annotation databases

EnsemblBacteriaiACM32311; ACM32311; Dtpsy_0833.
KEGGidia:Dtpsy_0833.
PATRICi21778698. VBIDiaSp55748_0860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM32311.1.
RefSeqiWP_012655803.1. NC_011992.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi535289.Dtpsy_0833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM32311; ACM32311; Dtpsy_0833.
KEGGidia:Dtpsy_0833.
PATRICi21778698. VBIDiaSp55748_0860.

Phylogenomic databases

eggNOGiENOG4105F91. Bacteria.
COG0061. LUCA.
HOGENOMiHOG000227221.
KOiK00858.
OMAiLAINCGR.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_ACIET
AccessioniPrimary (citable) accession number: B9ME57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 2, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.