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B9ME49 (ISPE_ACIET) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:Dtpsy_0825
OrganismAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY)) [Complete proteome] [HAMAP]
Taxonomic identifier535289 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2832834-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_1000190685

Regions

Nucleotide binding94 – 10411ATP Potential

Sites

Active site121 By similarity
Active site1361 By similarity

Sequences

Sequence LengthMass (Da)Tools
B9ME49 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: A0BD966CAA7B8BA2

FASTA28330,491
        10         20         30         40         50         60 
MQALYDVPAP AKLNLFLHVT GRRPDGYHLL QSVFMLIDWC DVLHFEVRGN GTVTREDLTS 

        70         80         90        100        110        120 
ALPADDLVVR AARALQKATG CPQGVHIGVS KHIPAQAGLG GGSSDAASTL LALNRLWKLK 

       130        140        150        160        170        180 
LSRQQLHSIA LTLGADVPFF LCGASAWVEG IGDIIRRLEL EHQLPPAAFA VVKPEAGLDT 

       190        200        210        220        230        240 
RMIFSHPSLK RDSSCATISG FAATHYQFGS NDLQPVAQAL CPEITQAINW LESKGLQGRM 

       250        260        270        280 
TGSGSAVFAQ ITHAVDLHDA PAAWHVKVCE NLKSHPLADW VFG 

« Hide

References

[1]"Complete sequence of Diaphorobacter sp. TPSY."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Coates J.D.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TPSY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001392 Genomic DNA. Translation: ACM32303.1.
RefSeqYP_002552303.1. NC_011992.1.

3D structure databases

ProteinModelPortalB9ME49.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING535289.Dtpsy_0825.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM32303; ACM32303; Dtpsy_0825.
GeneID7383520.
KEGGdia:Dtpsy_0825.
PATRIC21778682. VBIDiaSp55748_0852.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019601.
KOK00919.
OMAVSTAEIF.
OrthoDBEOG62VNQ2.
ProtClustDBPRK00343.

Enzyme and pathway databases

BioCycAEBR535289:GHOO-830-MONOMER.
UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_ACIET
AccessionPrimary (citable) accession number: B9ME49
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways