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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei12 – 121UniRule annotation
Active sitei136 – 1361UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 10411ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAEBR535289:GHOO-830-MONOMER.
UniPathwayiUPA00056; UER00094.

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:Dtpsy_0825
OrganismiAcidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY))
Taxonomic identifieri535289 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
ProteomesiUP000000450: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2832834-diphosphocytidyl-2-C-methyl-D-erythritol kinasePRO_1000190685Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi535289.Dtpsy_0825.

Structurei

3D structure databases

ProteinModelPortaliB9ME49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1947.
HOGENOMiHOG000019601.
KOiK00919.
OMAiYHNLQTL.
OrthoDBiEOG62VNQ2.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.

Sequencei

Sequence statusi: Complete.

B9ME49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALYDVPAP AKLNLFLHVT GRRPDGYHLL QSVFMLIDWC DVLHFEVRGN
60 70 80 90 100
GTVTREDLTS ALPADDLVVR AARALQKATG CPQGVHIGVS KHIPAQAGLG
110 120 130 140 150
GGSSDAASTL LALNRLWKLK LSRQQLHSIA LTLGADVPFF LCGASAWVEG
160 170 180 190 200
IGDIIRRLEL EHQLPPAAFA VVKPEAGLDT RMIFSHPSLK RDSSCATISG
210 220 230 240 250
FAATHYQFGS NDLQPVAQAL CPEITQAINW LESKGLQGRM TGSGSAVFAQ
260 270 280
ITHAVDLHDA PAAWHVKVCE NLKSHPLADW VFG
Length:283
Mass (Da):30,491
Last modified:March 24, 2009 - v1
Checksum:iA0BD966CAA7B8BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM32303.1.
RefSeqiYP_002552303.1. NC_011992.1.

Genome annotation databases

EnsemblBacteriaiACM32303; ACM32303; Dtpsy_0825.
GeneIDi7383520.
KEGGidia:Dtpsy_0825.
PATRICi21778682. VBIDiaSp55748_0852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001392 Genomic DNA. Translation: ACM32303.1.
RefSeqiYP_002552303.1. NC_011992.1.

3D structure databases

ProteinModelPortaliB9ME49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi535289.Dtpsy_0825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM32303; ACM32303; Dtpsy_0825.
GeneIDi7383520.
KEGGidia:Dtpsy_0825.
PATRICi21778682. VBIDiaSp55748_0852.

Phylogenomic databases

eggNOGiCOG1947.
HOGENOMiHOG000019601.
KOiK00919.
OMAiYHNLQTL.
OrthoDBiEOG62VNQ2.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094.
BioCyciAEBR535289:GHOO-830-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TPSY.

Entry informationi

Entry nameiISPE_ACIET
AccessioniPrimary (citable) accession number: B9ME49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.