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B9M9F3 (SYL_GEODF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Geob_2171
OrganismGeobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32) [Complete proteome] [HAMAP]
Taxonomic identifier316067 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199208

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9M9F3 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 03B7E84C731369F9

FASTA82493,327
        10         20         30         40         50         60 
MEEKYVPARV EEKWQKLWDS NKSFKAEKVE GKSKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VIARFKRMKG FNVLHPMGWD AFGMPAENAA IQHKSHPAKW TYENIDYMRG QLKKMGFSYD 

       130        140        150        160        170        180 
WDRELATCNV EYYKWEQLIF LQMLEKGLAY KKKSSVNWCP RCETVLANEQ VEDGSCWRCD 

       190        200        210        220        230        240 
SLVEQKELEQ WSFRITDYAE ELLEDTYKLP GWPERVLTMQ RNWIGRSTGC EIDFSIEGRK 

       250        260        270        280        290        300 
DAIKVFTTRQ DTLFGATFMS LAPEHPLALQ LTTAENMIVV NAFLDKVKKT DKIKRTAEDF 

       310        320        330        340        350        360 
EKEGVFTGSY CINPVTNRRM PIYLANFVLT DYGTGAVMAV PTHDQRDFEF ARKYAIAMEV 

       370        380        390        400        410        420 
VIQPEGGSLD VATMTEAYTA EGIMVNSGRF DGLNSAVAKE QIADFLEQEG LGKKTVNFRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPVIYC DDCGAVPVPA KDLPVVLPMD ATFTGEGGNP LSKIDSFIKT 

       490        500        510        520        530        540 
TCPLCGKDAR RETDTMDTFV ESSWYFLRYC CPDFACGPLD KGKTEYWMSV DQYIGGIEHA 

       550        560        570        580        590        600 
VMHLLYARFF TKVLRDLGYC DINEPFTNLL TQGMVIKDGS KMSKSKGNVV DPNALIEKYG 

       610        620        630        640        650        660 
ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLNRVWKLVY ECLPLISATG PLDAAALTDE 

       670        680        690        700        710        720 
GKTLRRLVHK TIRKVSDDIE DRFHFNTAIA AIMEMVNAIQ AFEPKNQPRN VPVLKEAVES 

       730        740        750        760        770        780 
VVLLLAPFVP HFAEELWESL GHEDNLNEAA WPAFDAAAAV DEELLVVVQV NGKLRGKVTV 

       790        800        810        820 
AASATDEDIK GAVLADEKIR QLIDGMNIKK IVYVPGKLVN IVVG 

« Hide

References

[1]"Complete sequence of Geobacter sp. FRC-32."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kostka J., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 22248 / JCM 15807 / FRC-32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001390 Genomic DNA. Translation: ACM20525.1.
RefSeqYP_002537626.1. NC_011979.1.

3D structure databases

ProteinModelPortalB9M9F3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316067.Geob_2171.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM20525; ACM20525; Geob_2171.
GeneID7363271.
KEGGgeo:Geob_2171.
PATRIC22010951. VBIGeoSp137169_2239.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGSP316067:GHSV-2196-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEODF
AccessionPrimary (citable) accession number: B9M9F3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries