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B9M4F4

- LIPA_GEODF

UniProt

B9M4F4 - LIPA_GEODF

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Protein
Lipoyl synthase
Gene
lipA, Geob_1320
Organism
Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi61 – 611Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGSP316067:GHSV-1335-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Geob_1320
OrganismiGeobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32)
Taxonomic identifieri316067 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000007721: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Lipoyl synthaseUniRule annotation
PRO_1000124633Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316067.Geob_1320.

Structurei

3D structure databases

ProteinModelPortaliB9M4F4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9M4F4-1 [UniParc]FASTAAdd to Basket

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MKITRRPEWL QKKVSPAAHA DMERLLGGLQ LHTVCQEAHC PNISECFRQR    50
QATFLILGKL CTRLCSFCNV TKQTPLAVDQ AEPERVAAAV ELLKLTHVVV 100
TSPTRDDLAD GGAALYAATV AAIRNASPQT KIELLVPDFA GNQESIAAVV 150
SACPHILGHN LETVPRLYSI RSGADYRRSL LMLEMIRRLN PAMKTKTGLM 200
LGLGETEEEL FQALRDLRRV DCSYLSLGQY LAPSRSHYPV QDYPSPETFD 250
RYREQALSMG FEHVESGPYV RSSYHAEHYG TGTGHAKLSP APAD 294
Length:294
Mass (Da):32,559
Last modified:March 24, 2009 - v1
Checksum:iEC8CC9CE0686C1AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001390 Genomic DNA. Translation: ACM19680.1.
RefSeqiYP_002536781.1. NC_011979.1.

Genome annotation databases

EnsemblBacteriaiACM19680; ACM19680; Geob_1320.
GeneIDi7364350.
KEGGigeo:Geob_1320.
PATRICi22009165. VBIGeoSp137169_1354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001390 Genomic DNA. Translation: ACM19680.1 .
RefSeqi YP_002536781.1. NC_011979.1.

3D structure databases

ProteinModelPortali B9M4F4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 316067.Geob_1320.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM19680 ; ACM19680 ; Geob_1320 .
GeneIDi 7364350.
KEGGi geo:Geob_1320.
PATRICi 22009165. VBIGeoSp137169_1354.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci GSP316067:GHSV-1335-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 22248 / JCM 15807 / FRC-32.

Entry informationi

Entry nameiLIPA_GEODF
AccessioniPrimary (citable) accession number: B9M4F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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