B9M1D2 (MDH_GEOSF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Geob_2664

Organism

Geobacter sp. (strain FRC-32) [Complete proteome] [HAMAP]

Taxonomic identifier

316067 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter › Geobacter daltonii

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_1000191648
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9M1D2 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: AC46F3790341EF60	FASTA	318	33,438
	10 20 30 40 50 60 MARKKIALIG GGQIGGVLAQ LSALRELGDV VLFDIVEGLP QGKTLDIAEA SPVDNFDAAL 70 80 90 100 110 120 TGANDYADIK GADIVIVTAG LPRKPGMSRD DLIATNAKIM QSVSEGIKQY APNSFVIVIS 130 140 150 160 170 180 NPLDAMVTLC QKITGFPSSR VMGMAGVLDS ARFAAFIAWE LGVSVKDVNA MVLGGHGDTM 190 200 210 220 230 240 VPIIRYANVN GVPVMELIER KYNGDKAKAK EVMAALVKRT QGAGGEVVGL LKTGSAFYSP 250 260 270 280 290 300 ASSAIAMAEA ILRDQKRLLP VCALLNGEFG VKGYYVGVPC ILGANGIEKI VEFSLDAEEQ 310 AMFDNSVAAV KELVDSMK

References

[1]

"Complete sequence of Geobacter sp. FRC-32." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kostka J., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FRC-32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001390 Genomic DNA. Translation: ACM21014.1.
RefSeq	YP_002538115.1. NC_011979.1.
3D structure databases
ProteinModelPortal	B9M1D2.
ModBase	Search...
Protein-protein interaction databases
STRING	316067.Geob_2664.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM21014; ACM21014; Geob_2664.
GeneID	7361075.
KEGG	geo:Geob_2664.
PATRIC	22011997. VBIGeoSp137169_2756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DANIVEC.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	GSP316067:GHSV-2695-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_GEOSF

Accession

Primary (citable) accession number: B9M1D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program