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Protein

Glutamate 5-kinase

Gene

proB

Organism
Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei154Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 175ATPUniRule annotation2
Nucleotide bindingi216 – 222ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Geob_0607
OrganismiGeobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32)
Taxonomic identifieri316067 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000007721 Componenti: Chromosome

Subcellular locationi

B9M0D5:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001936961 – 373Glutamate 5-kinaseAdd BLAST373

Interactioni

Protein-protein interaction databases

STRINGi316067.Geob_0607.

Structurei

3D structure databases

ProteinModelPortaliB9M0D5.
SMRiB9M0D5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 359PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiTHEIRFG.
OrthoDBiPOG091H00OF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR036974. PUA_sf.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9M0D5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKEILKKVK RVVIKIGSGV ITSVDNGLDG DRIKGLAAEM AHFRQQGVEV
60 70 80 90 100
VLVSSGAVAA GRLELGLSDR PRTIPQKQAA AAIGQSKLMR AYEEEFACHG
110 120 130 140 150
IKVAQLLLTR DDLASRPRFL NARATIDTLL ECGIIPIINE NDTVVVEEIK
160 170 180 190 200
FGDNDNLSAL VTNLVEAQLL VILTDIDGFY TADPRTNPEA RLIHLVRSIT
210 220 230 240 250
KETERAAGGS GSSVGTGGMA TKIAAAKKVG KSGIPTIMVN GKKSGNLELV
260 270 280 290 300
LAGTEVGTLF LPAGVSLNRR KHWIAYTQRP AGRVVVDNGA FRVLSQHGRS
310 320 330 340 350
LLPSGVVQVE GKFDRGACVR ICTPDGTEFA RGITDYSSRE MELIAGHKSG
360 370
DIEQILGYRY GDDVIHRDNL VVL
Length:373
Mass (Da):40,128
Last modified:March 24, 2009 - v1
Checksum:iFB367494D8D9FAC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001390 Genomic DNA. Translation: ACM18972.1.
RefSeqiWP_012645701.1. NC_011979.1.

Genome annotation databases

EnsemblBacteriaiACM18972; ACM18972; Geob_0607.
KEGGigeo:Geob_0607.

Similar proteinsi

Entry informationi

Entry nameiPROB_GEODF
AccessioniPrimary (citable) accession number: B9M0D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families