B9LUM0 (SYP_HALLT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Hlac_0777

Organism

Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) [Complete proteome] [HAMAP]

Taxonomic identifier

416348 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorubrum

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215564

Sequences

	Sequence		Length	Mass (Da)
	B9LUM0 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 089D1EBC845E8CFF	FASTA	491	54,939
	10 20 30 40 50 60 MSDDDQELGI TESKSHNTGE WYAEVVQKAG LADYGPEGMS GFIVTRPRAY AVWERLQGFL 70 80 90 100 110 120 DAKFKDTGVQ NAYFPLFIPE SYLEREKDIV EGFDPEVAWV TEAGNKELEE RLAVRPTSES 130 140 150 160 170 180 IITPYISQWV RSHRDLPLRV NQWCSVVRWE ATETKPFFRT KEFLWQEGHT AHATHEGAWE 190 200 210 220 230 240 ETMTRLDQYA SVYEDLLAMP VLKGQKPDHD KFPGAETTTT VEALMPDGKS VQAGTSHHLG 250 260 270 280 290 300 QSFAEAFDIT FSDEDEEERI AHTTSWGLSW RALGALIMTH SDEQGLVLPP GVAPEQVVVV 310 320 330 340 350 360 PIWQEDTKDE VLEYAEGVAD DLDDAGIRVE LDDRDGRNPG FKFNEHELNG VPLRIEIGPH 370 380 390 400 410 420 EVEDGELTLV HRPDGESVVE DREGVVATVQ DHFDEVYAKL YATAEETLDG AVREADDRAD 430 440 450 460 470 480 ILGTLGQHGG YVTAPWCGDE ACEEPIKEPM AAEIVMVPFE DDDPLAEADH GETCAICDDD 490 AERTAYFAKS Y

References

[1]

"Complete sequence of chromosome1 of Halorubrum lacusprofundi ATCC 49239." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Anderson I., DasSarma S., Cavicchioli R., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001365 Genomic DNA. Translation: ACM56377.1.
RefSeq	YP_002565447.1. NC_012029.1.
3D structure databases
ProteinModelPortal	B9LUM0.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LUM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7400252.
GenomeReviews	Gene locus Hlac_0777 in contig CP001365_GR.
KEGG	hla:Hlac_0777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_HALLT

Accession

Primary (citable) accession number: B9LUM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program