B9LUG4 (SYA_HALLT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Hlac_0719

Organism

Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) [Complete proteome] [HAMAP]

Taxonomic identifier

416348 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorubrum

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 926	926	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000198862
Sites
	Metal binding	615	1	Zinc By similarity
	Metal binding	619	1	Zinc By similarity
	Metal binding	719	1	Zinc By similarity
	Metal binding	723	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9LUG4 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 95304D4D9009714E	FASTA	926	102,340
	10 20 30 40 50 60 MSDLEAEYRL DYFEEEGFER KECPSCGAHF WTRDADRELC GEPPCEDYSF IDDPGFPEAY 70 80 90 100 110 120 SLSEMREAFL SFFEEHGHER IDPYPVAANR WRDDVLLTQA SIYDFQPLVT SGQTPPPANP 130 140 150 160 170 180 LTISQPCIRM QDIDNVGKTG RHTMAFEMMA HHAFNTREEV AEDEYAYHGE VYWKDETVEY 190 200 210 220 230 240 CDKLFESLGA DLEDITYIED PWVGGGNAGP AIEVIYKGAE LATLVFMCME RDPDGDYEMK 250 260 270 280 290 300 DGHTYSFMDT YIVDTGYGLE RWTWMSQGTA TVYEAVYPDA IDFLKENAGI EHTEAEQKLV 310 320 330 340 350 360 HRAAKLSGRL DIDDVDDVEA ARGEIADRLD VDVNRLRELV EPLESIYAIA DHSRTLAYMF 370 380 390 400 410 420 GDGIVPSNVG TGYLARMVLR RTKRLVDEIG IDAPLDELVD MQAERLGYEN RDTIREIVRT 430 440 450 460 470 480 EERKYRKTLE RGSRKVESLA DEYAGTDEPI PTEVLLELYD SHGIQPDMVA DIAAERGATV 490 500 510 520 530 540 DVPDDFYALV ADRHEEADGD EAAAERDDRF DDLPETEKLF YDDQGRTEFE AVVLDVFELE 550 560 570 580 590 600 EGYDVVLDQT MFYPEGGGQP ADRGQLTAGE TTVDVVDVQE RNGVVLHRTD ADPGKGEFVR 610 620 630 640 650 660 GQVDGDRRDR LRAHHTATHL IGHAAREVLG NHVRQAGAQK GIDSSRLDIR HFERITREQV 670 680 690 700 710 720 KEIERVANAL VRDDVPVRQE WPDRNEAESE HGFDLYQGGV PPGTNIRLIH VGDEDVQACA 730 740 750 760 770 780 GTHVERTGEI GAVKVLKTEP VQDGVERIVF AAGGAAVEAT QRTEDALYDA ARALDVDPLD 790 800 810 820 830 840 VPETAERFFE EWKGRGKEIE SLKEELAAAR ASGGADAEEV EIGGVTAVIQ RLDGDADELR 850 860 870 880 890 900 ATANAHVDDG KVAVVGSGAD GSASFVVGVP DGVDVNAGQV VSELAARVGG GGGGPPDFAQ 910 920 GGGPDADALD DALDAAPDVL RSLQEA

References

[1]

"Complete sequence of chromosome1 of Halorubrum lacusprofundi ATCC 49239." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Anderson I., DasSarma S., Cavicchioli R., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001365 Genomic DNA. Translation: ACM56321.1.
RefSeq	YP_002565391.1. NC_012029.1.
3D structure databases
ProteinModelPortal	B9LUG4.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LUG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7400192.
GenomeReviews	Gene locus Hlac_0719 in contig CP001365_GR.
KEGG	hla:Hlac_0719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MFTNSGM.
ProtClustDB	PRK13902.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_HALLT

Accession

Primary (citable) accession number: B9LUG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program