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B9LU22 (DNLJ_HALLT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:Hlac_2645
OrganismHalorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) [Complete proteome] [HAMAP]
Taxonomic identifier416348 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorubrum

Protein attributes

Sequence length710 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 710710DNA ligase HAMAP MF_01588
PRO_0000380393

Regions

Domain623 – 71088BRCT
Nucleotide binding63 – 675NAD By similarity
Nucleotide binding111 – 1122NAD By similarity

Sites

Active site1491N6-AMP-lysine intermediate By similarity
Metal binding4441Zinc By similarity
Metal binding4471Zinc By similarity
Metal binding4601Zinc By similarity
Metal binding4661Zinc By similarity
Binding site1471NAD By similarity
Binding site1701NAD By similarity
Binding site2061NAD By similarity
Binding site3531NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
B9LU22 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: BCD1D8DA180D111A

FASTA71077,226
        10         20         30         40         50         60 
MTSSSPRHAD PDENPYVEAP PTDFEPVGAL SEDEATEQAS LLRAAIREHD HRYYLEADPL 

        70         80         90        100        110        120 
IPDETYDRLF TRLQELENEF DLPTQNSPTR RVGGEPLDEL ATVEHVAPMR SIDNATEADA 

       130        140        150        160        170        180 
VREFDGRVRK GLDAEGFDSD AVEYVCEPKF DGLSVEVIYE DGEYVRAATR GDGTAGDDVT 

       190        200        210        220        230        240 
EQVRTIRSVP GKLRGDPPSR LAVRGEAYMP RDAFKAYNEA LMERGEEPFA NPRNAAAGTL 

       250        260        270        280        290        300 
RQLDPSVVAE RPLDIFFFDV LGWENDAEGD SPNRPATHWE EFDTFDAFGL RRANRVERVD 

       310        320        330        340        350        360 
DIEGALDYRD RLMADREDLN FAIDGVVIAV DDRANREALG ATARAPRWAF AYKFPPRTAT 

       370        380        390        400        410        420 
TIVEGITVQV GRTGRLTPVA ELDPIDVGGV TVSRATLHNP AEIEALGVNV GDCVRIYRAG 

       430        440        450        460        470        480 
DVIPYVPEVV EKRSEGTYVF PETCPICDAP VERDGPLAFC TGGLVCPAQL ERAVEHWARR 

       490        500        510        520        530        540 
DALDIEGLGP ERVQQLREAG LVESLPDLYD LAVDDLAALE GWGETSAENL IAELAATRDP 

       550        560        570        580        590        600 
PLDDFLAGLG IPDVGATTAR ALAAHFGDLD AILDADEDAL RAVDDVGPEV AESIRTFLDN 

       610        620        630        640        650        660 
AENRVAIDGL RERGVDPESV DVETGDALDG LTFVFTGSLS TTRGEAQAHV EAHGADATSS 

       670        680        690        700        710 
VSGNTDYLVA GESPGRSKRD DADAEGVPVV DEEEFAGLLA ERGVAWPPEE

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References

[1]"Complete sequence of chromosome1 of Halorubrum lacusprofundi ATCC 49239."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. expand/collapse author list , Anderson I., DasSarma S., Cavicchioli R., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001365 Genomic DNA. Translation: ACM58216.1.
RefSeqYP_002567286.1. NC_012029.1.

3D structure databases

ProteinModelPortalB9LU22.
ModBaseSearch...

Protein-protein interaction databases

STRINGB9LU22.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7400850.
GenomeReviewsGene locus Hlac_2645 in contig CP001365_GR.
KEGGhla:Hlac_2645.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAENVRTIR.
ProtClustDBPRK14351.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 4 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. False negative.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_HALLT
AccessionPrimary (citable) accession number: B9LU22
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 16, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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