B9LTX1 (GUAAB_HALLT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Hlac_2593

Organism

Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) [Complete proteome] [HAMAP]

Taxonomic identifier

416348 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorubrum

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000133393
Regions
	Domain	2 – 185	184	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9LTX1 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: CB4F65A2E4EC6FA4	FASTA	305	33,646
	10 20 30 40 50 60 MVETEEFIAE AKAEIREAIG DANAVIALSG GVDSSVAATL AYEAVGDQLT PVYVDTGLMR 70 80 90 100 110 120 KGETEEIRET FSFMESLRVI EAQERFFDRL AGVTDPEEKR HVIGEGFIDE FETVANDVGA 130 140 150 160 170 180 DYLVQGTIYP DRIESEGNIK SHHNVGGLPD IVDFEGIVEP VRDLYKDEVR EVARALGLEE 190 200 210 220 230 240 VISERMPFPG PGLAVRIVGE VTPEKAAVAR EATHVVEEEL EEYDPWQAFA AVLGKATGVK 250 260 270 280 290 300 GDNRVHGWVV AVRSVESRDG MTARAQELDW STLQRIQSRI TGENENVARV VYDVTHKPPA TIEYE

References

[1]

"Complete sequence of chromosome1 of Halorubrum lacusprofundi ATCC 49239." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Anderson I., DasSarma S., Cavicchioli R., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001365 Genomic DNA. Translation: ACM58165.1.
RefSeq	YP_002567235.1. NC_012029.1.
3D structure databases
ProteinModelPortal	B9LTX1.
SMR	B9LTX1. Positions 4-305.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LTX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7399818.
GenomeReviews	Gene locus Hlac_2593 in contig CP001365_GR.
KEGG	hla:Hlac_2593.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_HALLT

Accession

Primary (citable) accession number: B9LTX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program