B9LT08 (PURL_HALLT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Hlac_0471

Organism

Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) [Complete proteome] [HAMAP]

Taxonomic identifier

416348 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halorubrum

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000134899
Regions
	Nucleotide binding	108 – 119	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B9LT08 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 50EC737C76F461D1	FASTA	759	78,583
	10 20 30 40 50 60 MSLSEPDHEL VVAELGREPT AAEVALFENL WSEHCAYRSS RPLLSAFESE GDQVVVGPGD 70 80 90 100 110 120 DAAVLALPEP DAADTPAAER DADDYGDQYV TFGVESHNHP SFVDPVDGAA TGVGGIVRDT 130 140 150 160 170 180 MSMGAYPIAL LDSLYFGGFD RERSRYLFEG VVEGISHYGN CIGVPTVGGS VAFHDGYEGN 190 200 210 220 230 240 PLVNVACVGL TNEDRLVTAT AQEPGNTLML VGNGTGRDGL GGASFASEDL AEDAETEDRP 250 260 270 280 290 300 AVQVGDPYAE KRLIECNEAL VDEDLVLSAR DLGAAGLGGA SSELVAKGGL GARLDLDSVH 310 320 330 340 350 360 QREPNMNAME ILLAESQERM CYEVAPEDVA RVEALAERFD LGCSVIGEVT DGNYVCEFAG 370 380 390 400 410 420 AGKGEDDADD SDAESEVVVD VDAEYLADGA PMNDLASESP TQPDRDLPDP EPSLDEAVES 430 440 450 460 470 480 VVSAPSTASK RWVYRQYDHE VGVRTAMKPG DDAAIMAIRE TASTDAADLA PADQGVGLAL 490 500 510 520 530 540 SSGANPNWTE TDPYEGARAV ALENATNLAA KGAVPLAAVD CLNGGNPEKP EVYGGFKGIV 550 560 570 580 590 600 DGLADACADL DAPVVGGNVS LYNDSVEGPI PPTPTLALIG TRKGYNAPPA ALDADSAGDS 610 620 630 640 650 660 ELLLIGAGGA DGGALGGSEY LAQAGGTDRF PTLPDTETKS LADRVASLAA VARHESTFAT 670 680 690 700 710 720 HDVSDGGLAV ALAELVTDAA GADVTLPDRV AVFEETPGRL VVQTTDPEAV AELAGELPVL 730 740 750 RLGEVTTDGA LSLSVGDDET AFDADTIREL RGVIDRELA

References

[1]

"Complete sequence of chromosome1 of Halorubrum lacusprofundi ATCC 49239." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N. , Anderson I., DasSarma S., Cavicchioli R., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001365 Genomic DNA. Translation: ACM56073.1.
RefSeq	YP_002565143.1. NC_012029.1.
3D structure databases
ProteinModelPortal	B9LT08.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LT08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7400351.
GenomeReviews	Gene locus Hlac_0471 in contig CP001365_GR.
KEGG	hla:Hlac_0471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_HALLT

Accession

Primary (citable) accession number: B9LT08

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program