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Protein

Enolase

Gene

eno

Organism
Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (Hlac_0562)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Active sitei195Proton donorUniRule annotation1
Metal bindingi231MagnesiumUniRule annotation1
Metal bindingi274MagnesiumUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Metal bindingi301MagnesiumUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Active sitei326Proton acceptorUniRule annotation1
Binding sitei326Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei377SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciHLAC416348:GIWW-1871-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Hlac_1825
OrganismiHalorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)
Taxonomic identifieri416348 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHalorubrum
Proteomesi
  • UP000000740 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001899601 – 400EnolaseAdd BLAST400

Interactioni

Protein-protein interaction databases

STRINGi416348.Hlac_1825.

Structurei

3D structure databases

ProteinModelPortaliB9LPW6.
SMRiB9LPW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 356Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiDQTLICG.
OrthoDBiPOG093Z03D9.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

B9LPW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRITGISLR RVLDSRGNPT VEADVLTESG GFGRGAAPSG ASTGEYEAIE
60 70 80 90 100
LPANESIAKA REHAVPRLEG VYAGDQRAVD NALRAADGTD DFSAIGANSA
110 120 130 140 150
VAISMAAAKA AADVLGAPLY QHLGGAFRGE NFPIPLGNVV GGGEHAKEAT
160 170 180 190 200
HIQEFLAAPV GAPSVSEAVF ANAAVHAAVA DVLDERGVPA AKGDEGAWAP
210 220 230 240 250
PISDADAFEV VDEAVDRVEE DVGFEIRFGL DMAAAELYDD DQEAYVYGEE
260 270 280 290 300
TKSTDEQIDY VADLVDEYDL AYVEDPLDEN DYEAFAELTD RVGDRTMICG
310 320 330 340 350
DDLFVTNVER LQEGIDTGAA NSILIKPNQI GTLSDTFDAI ELAARNGYET
360 370 380 390 400
IISHRSGETE DTTIAHLAVA TDAGFIKTGT VGGERTAKLN ELVRIADDAV
Length:400
Mass (Da):42,081
Last modified:March 24, 2009 - v1
Checksum:iCFC7A348F9FFC7F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001365 Genomic DNA. Translation: ACM57404.1.
RefSeqiWP_015910538.1. NC_012029.1.

Genome annotation databases

EnsemblBacteriaiACM57404; ACM57404; Hlac_1825.
GeneIDi7400017.
KEGGihla:Hlac_1825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001365 Genomic DNA. Translation: ACM57404.1.
RefSeqiWP_015910538.1. NC_012029.1.

3D structure databases

ProteinModelPortaliB9LPW6.
SMRiB9LPW6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416348.Hlac_1825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM57404; ACM57404; Hlac_1825.
GeneIDi7400017.
KEGGihla:Hlac_1825.

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiDQTLICG.
OrthoDBiPOG093Z03D9.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciHLAC416348:GIWW-1871-MONOMER.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_HALLT
AccessioniPrimary (citable) accession number: B9LPW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: March 15, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.