B9LKW0 (FTHS_CHLSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Chy400_2847

Organism

Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

480224 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000185250
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9LKW0 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 25EFDFB2BF369EF9	FASTA	572	60,580
	10 20 30 40 50 60 MKTSLQIAAE ARLEPIAAIA ERLGLPVRYL EPYGRYRGKI DLTFLDDYHD RPLGRYVLVS 70 80 90 100 110 120 AITPTPLGEG KTTTAIGLAM ALNRIGKRAA VTLRQSSLGP VFGIKGGGAG GGYSQIVPLV 130 140 150 160 170 180 ESILHLNGDI HAVSQAHNQL AALTDNSWYH GNPLDIDPDR IEIRRVVDVN DRFLRQVMIG 190 200 210 220 230 240 LGGKQNGFPR QTGFDISVAS ELMAILAMVN GVGARAALRD LRSRIGRMVV AFRRDGTPIT 250 260 270 280 290 300 AEDVRGAGAA TVLMREALKP NLMQTIENTP ALIHAGPFAN IAQGNSSILA DLIALRCADY 310 320 330 340 350 360 VVTEAGFGVD IGAEKFFNLK CRASGLWPDV AVIVATIRAL KAHSGKYDIV AGKPLPPALL 370 380 390 400 410 420 HENPDDVISG GANLRRQIEN LHQFKVPVIV ALNAYPEDTP AEIDAVAHIA TTAGAAGMAV 430 440 450 460 470 480 SNVYAAGSAG GVDLARLVIE IAERPGPRPV QFLYPLEWSL ADKITTIAHR IYGAAAVTFS 490 500 510 520 530 540 PTAAAQLAAL EDAGFGNLPI CMAKTHLSLS HDPALRGAPE GFTFPIREVR LSAGAGFILP 550 560 570 IAGTTVTMPG LGAHPAAHQI DIDDEGNIVG LF

References

[1]

"Complete sequence of Chloroflexus sp. Y-400-fl." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Bryant D.A., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001364 Genomic DNA. Translation: ACM54235.1.
RefSeq	YP_002570561.1. NC_012032.1.
3D structure databases
ProteinModelPortal	B9LKW0.
SMR	B9LKW0. Positions 5-570.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LKW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7404319.
GenomeReviews	Gene locus Chy400_2847 in contig CP001364_GR.
KEGG	chl:Chy400_2847.
PATRIC	21425345. VBIChlSp61043_2951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NHLHHGN.
ProtClustDB	CLSK983690.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CHLSY

Accession

Primary (citable) accession number: B9LKW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program