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B9LKI7 (B9LKI7_CHLSY) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Chy400_0814 EMBL ACM52243.1
OrganismChloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP] EMBL ACM52243.1
Taxonomic identifier480224 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeChloroflexus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain143 – 348206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding175 – 23056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3011Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B9LKI7 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 74FAC4051A21905A

FASTA35637,791
        10         20         30         40         50         60 
MTDSITVAVV FGGQSGEHEV SLVSAHAVMS NLDPARYQIE AIGIGKDGQW WHGPGALAKL 

        70         80         90        100        110        120 
MAEADPARLP ASAAQVAAGP VHALGRVGAP GWELPAADVI FPVLHGPYGE DGTIQGLLEL 

       130        140        150        160        170        180 
AGRPYVGCGV AASAVGMDKG LMKAAFAAAG LPVLPWVLVR RSELADESGL CARLEAALRY 

       190        200        210        220        230        240 
PMFVKPANLG SSVGVSKVRD RAELLAGLHE AARYDRRLIV EQGIPAREIE ISVLGNDEVV 

       250        260        270        280        290        300 
ASIPGEIIPA GEWYDYHAKY IDGGSQTIVP ADLSPAQITQ VQELAMRAFQ AIDGAGLARV 

       310        320        330        340        350 
DFLLDRDQGT LWVNEVNTMP GFTPISMYPK MWAASGLSYA QLLDRLVDLA LERARR 

« Hide

References

[1]"Complete sequence of Chloroflexus sp. Y-400-fl."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ovchinnikova G., Bryant D.A., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001364 Genomic DNA. Translation: ACM52243.1.
RefSeqYP_002568569.1. NC_012032.1.

3D structure databases

ProteinModelPortalB9LKI7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING480224.Chy400_0814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM52243; ACM52243; Chy400_0814.
GeneID7405320.
KEGGchl:Chy400_0814.
PATRIC21421069. VBIChlSp61043_0846.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBCLSK974977.

Enzyme and pathway databases

BioCycCSP480224:GHIY-825-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9LKI7_CHLSY
AccessionPrimary (citable) accession number: B9LKI7
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)