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B9LHK4

- PFKA_CHLSY

UniProt

B9LHK4 - PFKA_CHLSY

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi116 – 1161Magnesium; catalyticUniRule annotation
    Sitei117 – 1171Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei140 – 1401Proton acceptorUniRule annotation
    Binding sitei175 – 1751Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei235 – 2351SubstrateUniRule annotation
    Binding sitei272 – 2721Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 792ATPUniRule annotation
    Nucleotide bindingi115 – 1184ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCSP480224:GHIY-277-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Chy400_0274
    OrganismiChloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
    Taxonomic identifieri480224 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
    ProteomesiUP000007725: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356ATP-dependent 6-phosphofructokinasePRO_1000192368Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi480224.Chy400_0274.

    Structurei

    3D structure databases

    ProteinModelPortaliB9LHK4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni138 – 1403Substrate bindingUniRule annotation
    Regioni182 – 1843Substrate bindingUniRule annotation
    Regioni278 – 2814Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiHRHRAHA.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9LHK4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASGLGWEVIG IHDGFEGLLG    50
    TKSYRVLTNA DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK 100
    AIEEMGLRAL ITIGGEGTQR IALELHKLGA PVIGVPKTID NDLAGTDRTF 150
    GFDTALQVAT DAIDRLHTTA ASHNRVMVLE VMGRHTGWIA LHAGLAGGAD 200
    VILIPEIPFS IERVAEKVMA RDQQGSSFSI IVVAEGARPR GGSEMYIAEG 250
    RLGGIGHWVG EQLEKLTAKE VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA 300
    VQAAARGIYG EMVALRGQDI VTVPLAEACG HLNRVRPHSD LVLCARSLGI 350
    AFGDEL 356
    Length:356
    Mass (Da):37,703
    Last modified:March 24, 2009 - v1
    Checksum:iC8B7B858A07B643C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001364 Genomic DNA. Translation: ACM51713.1.
    RefSeqiYP_002568038.1. NC_012032.1.

    Genome annotation databases

    EnsemblBacteriaiACM51713; ACM51713; Chy400_0274.
    GeneIDi7402994.
    KEGGichl:Chy400_0274.
    PATRICi21419952. VBIChlSp61043_0295.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001364 Genomic DNA. Translation: ACM51713.1 .
    RefSeqi YP_002568038.1. NC_012032.1.

    3D structure databases

    ProteinModelPortali B9LHK4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 480224.Chy400_0274.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM51713 ; ACM51713 ; Chy400_0274 .
    GeneIDi 7402994.
    KEGGi chl:Chy400_0274.
    PATRICi 21419952. VBIChlSp61043_0295.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi HRHRAHA.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci CSP480224:GHIY-277-MONOMER.

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29364 / DSM 637 / Y-400-fl.

    Entry informationi

    Entry nameiPFKA_CHLSY
    AccessioniPrimary (citable) accession number: B9LHK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3