B9LHB5 (B9LHB5_CHLSY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACM55588.1
EC=5.99.1.2 EMBL ACM55588.1

Gene names

Ordered Locus Names:

Chy400_4234

Organism

Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP] EMBL ACM55588.1

Taxonomic identifier

480224 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B9LHB5 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 2D83AA4C94391273	FASTA	776	86,765
	10 20 30 40 50 60 MGEKVVIVES PAKARTIQKY LGKGYKVASS MGHVRDLPKS GLAIDIEHDF APSYEIVKPK 70 80 90 100 110 120 VVSELSQALR NAEAVYLATD PDREGEAIAW HITQAVKLPK KTPVYRVVFQ EITRNAVQQA 130 140 150 160 170 180 LQQPRQINQN LVEAQQARRV LDRLVGYQLS PLLWDKVKRG LSAGRVQSVA VRLIVERERE 190 200 210 220 230 240 IEQFQPQEYW TIEADLLKDA GVAPRDLFRA VLIERDGKKL EKFSIENREQ AEAIVADLQG 250 260 270 280 290 300 AAYTVVRVTR RDKRRSPAPP FTTSTLQQEA ARKLGFSAKK TMTLAQRLYE GVDIGGEDGM 310 320 330 340 350 360 VGLITYMRTD SVQVAAEAQT EARDVIGKRF GKEYLPDQPP VYKTKAKGAQ EAHEAIRPTS 370 380 390 400 410 420 SARLPEQLSG KLENDLWRLY DLIWKRFIAS QMAPAVFDST TVDIAATPVV AGAPAYLFRA 430 440 450 460 470 480 TGSVLKFPGF LAVYNVSLDE GEEDEDSERR LPPLAEGEAL QLVELLPIQH FTEPPPRYTE 490 500 510 520 530 540 ASLVKELERL GIGRPSTYAS ILSTIQEREY VEMVDKKLIP TTLGRVVTDL LVEHFGNIVD 550 560 570 580 590 600 YDFTSALEQQ LDDIAEGSKK WVPVLREFYG PFRSTLEQAQ RQMRNVKREE IVTDLDCPKC 610 620 630 640 650 660 GQGKLVIRFG RNGEFLACSR YNREGDGESC DFTSDFHRDE QGQIVIDKAS APETSDVLCN 670 680 690 700 710 720 VCGRPMVIKK SRFGPFLGCS GYPECNNTRR IGRDGKPVPL PEPTGVQCPK CGEGELLRRR 730 740 750 760 770 GKFGRPFYGC SRYPKCDYIT NTLDEAQAGE APAEPAKNEP ALPSPSRSSK RTRKSA

References

[1]

"Complete sequence of Chloroflexus sp. Y-400-fl." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Bryant D.A., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001364 Genomic DNA. Translation: ACM55588.1.
RefSeq	YP_002571914.1. NC_012032.1.
3D structure databases
ProteinModelPortal	B9LHB5.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LHB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7406148.
GenomeReviews	Gene locus Chy400_4234 in contig CP001364_GR.
KEGG	chl:Chy400_4234.
PATRIC	21428304. VBIChlSp61043_4414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B9LHB5_CHLSY

Accession

Primary (citable) accession number: B9LHB5

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)