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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931MagnesiumUniRule annotation
Metal bindingi499 – 4991MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCSP480224:GHIY-2237-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Chy400_2208
OrganismiChloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)
Taxonomic identifieri480224 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
ProteomesiUP000007725: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 755755Polyribonucleotide nucleotidyltransferasePRO_0000381875Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi480224.Chy400_2208.

Structurei

3D structure databases

ProteinModelPortaliB9LH03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 61960KHUniRule annotationAdd
BLAST
Domaini629 – 69870S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9LH03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTERNIYSVS AEIAGRTLTL EAGRFAEQAD GAVVARYGDT MLLATVVCAK
60 70 80 90 100
EAREGTDFFP LTVDYEEKMY AVGKIPGNFF KREGRPTTTA ILISRLTDRP
110 120 130 140 150
LRPLFPKGFY NEVQVIITTF SIDMENDPGP LAIIAASAAL CISDIPFAGP
160 170 180 190 200
VGAVQMGHLN GQLVVNPKMN EIADSRLDLV VAGTKDAVLM VEAGAYELTE
210 220 230 240 250
DEMLQAVIDG HAVCKQICDL QEQLVQLCGK PKRPFTPPVV DTSLEEAISA
260 270 280 290 300
WMGDRLRKAV RSPIKQEREA QTEALKAEVI AHFTADEPEE EIANRTKEVT
310 320 330 340 350
KAFEKLLKDE VRNAILDEGI RVDGRALDEI RPISIEVGVI PRVHGSAVFT
360 370 380 390 400
RGQTQVLTIT TLGSPGDEQK VDDLGIETSK RYIHHYNFPP FSTGEVRRIG
410 420 430 440 450
TPRRRDIGHG ALAERSLYAV LPDEKDFPYT IRLVSEVLSS NGSSSMASVC
460 470 480 490 500
GSSLSLMDAG VPIKAPVAGV AMGLITGEDG RWRVLTDIQG LEDALGDMDF
510 520 530 540 550
KVAGTAKGVT GLQMDIKTTG ITYEIMREAF AQARAGRLFI LDKMNEVISA
560 570 580 590 600
PRPELSIYAP RIMTIQIPVD KIGALIGPGG KTIRNICETT GAQIDIEDDG
610 620 630 640 650
RVFITTPDGA AARQAISMIE GLTREAKVGD IFLGKVVSIK PFGAFVNILP
660 670 680 690 700
GKDGMVHVSE LDEKRVENVE DVVSLGDEIN VMVIDIDRTT GKISLSRRAV
710 720 730 740 750
LTGETPEERK AAGAAPRPRP REEQRGGRDE PRSLRDELRG PRREGDRPRP

RRRDD
Length:755
Mass (Da):82,432
Last modified:March 24, 2009 - v1
Checksum:iD293AA822551BDCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001364 Genomic DNA. Translation: ACM53605.1.
RefSeqiYP_002569931.1. NC_012032.1.

Genome annotation databases

EnsemblBacteriaiACM53605; ACM53605; Chy400_2208.
GeneIDi7405523.
KEGGichl:Chy400_2208.
PATRICi21424033. VBIChlSp61043_2309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001364 Genomic DNA. Translation: ACM53605.1.
RefSeqiYP_002569931.1. NC_012032.1.

3D structure databases

ProteinModelPortaliB9LH03.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi480224.Chy400_2208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM53605; ACM53605; Chy400_2208.
GeneIDi7405523.
KEGGichl:Chy400_2208.
PATRICi21424033. VBIChlSp61043_2309.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciCSP480224:GHIY-2237-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Entry informationi

Entry nameiPNP_CHLSY
AccessioniPrimary (citable) accession number: B9LH03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.