B9LE47 (PROA_CHLSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Chy400_1753

Organism

Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

480224 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP-Rule MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP-Rule MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00412		PRO_1000193586

Sequences

	Sequence		Length	Mass (Da)
	B9LE47 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 68BC84715CCE3378	FASTA	422	44,515
	10 20 30 40 50 60 MVDLEAIGRR AKTAARALAK LSTEQKNAAL CAIADGLLAR QDKILAANAA DVADAEKGGT 70 80 90 100 110 120 PPAIVDRMLL TPARLAAIAG DCRQVASLPD PVGEIFDRRE LPSGLRLYKR RVPIGVIGAI 130 140 150 160 170 180 YEARPNVTVD IASLCLKAGN AVILRGGSDI ARSVAATTEV IALALEQAGL PAFAVQSIID 190 200 210 220 230 240 PDRELVRQLL RLDRYVDMII PRGGAGLHRF CVENATVPVI VGGMGVSHIY VEPSADFARA 250 260 270 280 290 300 VPVIVNAKVQ RPGACNALDT LLVHRAAAST FLPLVAAALA QHGVELRCDL EALAILADAP 310 320 330 340 350 360 GHEAWNLKPA SPTDFGCEFL ALIVAIKIVG SIDEALDHIA LYGGHSEAIL TGDPISAERF 370 380 390 400 410 420 TREVDATAVF VNASTRFNDG GQFGLGAEVA ISTNRLHARG PMGLQELTTY TWIGEGDYLV RA

References

[1]

"Complete sequence of Chloroflexus sp. Y-400-fl." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Bryant D.A., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001364 Genomic DNA. Translation: ACM53163.1.
RefSeq	YP_002569489.1. NC_012032.1.
3D structure databases
ProteinModelPortal	B9LE47.
ModBase	Search...
Protein-protein interaction databases
STRING	480224.Chy400_1753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM53163; ACM53163; Chy400_1753.
GeneID	7405142.
KEGG	chl:Chy400_1753.
PATRIC	21423067. VBIChlSp61043_1833.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HOG000246356.
KO	K00147.
OMA	EVGISTQ.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	CSP480224:GHIY-1773-MONOMER.
UniPathway	UPA00098; UER00360.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
HAMAP	MF_00412. ProA.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
PANTHER	PTHR11063:SF1. PTHR11063:SF1. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CHLSY

Accession

Primary (citable) accession number: B9LE47

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program