B9LDI5 (B9LDI5_CHLSY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022

Gene names

Name:	gltX HAMAP MF_00022
Ordered Locus Names:	Chy400_1668

Organism

Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP] EMBL ACM53079.1

Taxonomic identifier

480224 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 EMBL ACM53079.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	14 – 24	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	261 – 265	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	264	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B9LDI5 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 4666A8FA4194733F	FASTA	503	56,742
	10 20 30 40 50 60 MSSIEGPVRV RFAPSPTGSL HIGGVRTALF NWLCARHYGG QFILRIEDTD EKRFVPGAAD 70 80 90 100 110 120 DISASLRWVG IDWDEGPDVG GPYGPYVQSE RFEQGIYQPF INQLLEAGLA YMSFTTEEEL 130 140 150 160 170 180 AQMRAAAEAA GIKAFRFRGP ERDWPLERQR EEAASGKPYT IRLKTPLEGE TRFRDLIRGG 190 200 210 220 230 240 DEIVVQNNQL QDIVLIKSTG MPVYHFAHLV DDHLMKITHV MRGEEWVPST PYHVLLYDFF 250 260 270 280 290 300 GWPRPVFAHL PAILRQDGRG KLSKRKDDVA TQRFRERGYL PETIFNYLAL QGWSYDGVTE 310 320 330 340 350 360 IMTRDELIAR FTLERIQPSP ARWNPEKLRD MNGIYIRKLT TEQLAERVLP FMQRAGLIGD 370 380 390 400 410 420 PASETERAYL ISLIPLIHER LEELQEAPDL LAFFYQDVVP GETYNPADLI PKKHDAAQTV 430 440 450 460 470 480 ALLRAAHEAL SQQTDWTPPA LETTLRALCE QLQVKPGPLF GAIRVAITGR SVAPPLFDTL 490 500 AGVGRERSLS RLAAAIAALE NLA

References

[1]

"Complete sequence of Chloroflexus sp. Y-400-fl." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Bryant D.A., Richardson P. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001364 Genomic DNA. Translation: ACM53079.1.
RefSeq	YP_002569405.1. NC_012032.1.
3D structure databases
ProteinModelPortal	B9LDI5.
ModBase	Search...
Protein-protein interaction databases
STRING	B9LDI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7406888.
GenomeReviews	Gene locus Chy400_1668 in contig CP001364_GR.
KEGG	chl:Chy400_1668.
PATRIC	21422851. VBIChlSp61043_1725.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B9LDI5_CHLSY

Accession

Primary (citable) accession number: B9LDI5

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)