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B9LAP2 (NADK_CHLSY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Chy400_1167
OrganismChloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) [Complete proteome] [HAMAP]
Taxonomic identifier480224 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeChloroflexus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276NAD kinase HAMAP-Rule MF_00361
PRO_1000133563

Regions

Nucleotide binding61 – 622NAD By similarity
Nucleotide binding135 – 1362NAD By similarity

Sites

Active site611Proton acceptor By similarity
Binding site661NAD By similarity
Binding site1461NAD By similarity
Binding site1631NAD By similarity
Binding site1651NAD By similarity
Binding site2001NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B9LAP2 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: D0126CA5F2F1B11A

FASTA27630,253
        10         20         30         40         50         60 
MLERVAVLYN PLSDASIKLS RELADWLVER GVKTTRGVSQ EFRDQPHLVA DCDLMIALGG 

        70         80         90        100        110        120 
DGTVLRAARL CFPHNIPVLP VALGHLSFMA EIGPDEVYSG CEQIMNGGGW FDERSLVRAQ 

       130        140        150        160        170        180 
LWRGGQKLSQ HTALNEVVIS RSDLSRIVNV HVTIDDSPLT TYHADGVIVA TATGSTAYAL 

       190        200        210        220        230        240 
AAGGPIVDPR SQALVLVPIA AHLTNIPSMV LHEDAVVTMQ LRSRHHALLA VDGRENIDLI 

       250        260        270 
EGDEVVVRRS PQVCTFVRLR PSNQFYTQLV ARLRRS 

« Hide

References

[1]"Complete sequence of Chloroflexus sp. Y-400-fl."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Ovchinnikova G., Bryant D.A., Richardson P.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29364 / DSM 637 / Y-400-fl.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001364 Genomic DNA. Translation: ACM52588.1.
RefSeqYP_002568914.1. NC_012032.1.

3D structure databases

ProteinModelPortalB9LAP2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING480224.Chy400_1167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM52588; ACM52588; Chy400_1167.
GeneID7405756.
KEGGchl:Chy400_1167.
PATRIC21421805. VBIChlSp61043_1206.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMARWEALCL.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycCSP480224:GHIY-1184-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_CHLSY
AccessionPrimary (citable) accession number: B9LAP2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: July 9, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families