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B9L8Z9

- SYI_NAUPA

UniProt

B9L8Z9 - SYI_NAUPA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNPRO598659:GH7N-694-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NAMH_0696
OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Taxonomic identifieri598659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
ProteomesiUP000000448: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Isoleucine--tRNA ligasePRO_1000189183Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi598659.NAMH_0696.

Structurei

3D structure databases

ProteinModelPortaliB9L8Z9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9L8Z9 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDYKDTLLLP KTTFPMRGNL PQNEPKKYKK WFSEHVYERM KQNRVGNDKF
60 70 80 90 100
NLHDGPPYAN GHIHIGHALN KILKDMIVKY YYFQGFDVRY TPGWDCHGLP
110 120 130 140 150
IEQQVEKKIG REKKESLPKS KVRELCRQHA AKFIEIQKEE FQNLGVIGDW
160 170 180 190 200
DNPYKTMDFE FEANIYKALA EIAKKGLLVE RSKPVFWCMH DKTALAEAEV
210 220 230 240 250
EYEDKEDYSI YVAFPLSNEA KSKLGIDNAS IVIWTTTPWT LPANMGIALN
260 270 280 290 300
PEEKYVLSED GKIVAKELYE NLKEAEVVSG EIVKEFDASE LENLKAINPL
310 320 330 340 350
NGRESVIILG EHVTMDGGTG CVHTAPGHGE EDYRVWLKYG FSEILQPVDD
360 370 380 390 400
EGKYSNLIVT EKLLPEEFKG MHIFEANPKI LDLLGDNLVK VSKFTHSYPH
410 420 430 440 450
CWRCHNPVIF RATKQFFIAM DKEVNGDTLR HRALSEIEKV EFTPKTGKNR
460 470 480 490 500
LSTMVANRPD WCISRQRDWG VPIAFFRNKD TGELIIDDEI IENVYEIFKV
510 520 530 540 550
KGADAWYDLS IEELLPESKK EMASKLEKVN DILDVWFDSG STWFAVLKNG
560 570 580 590 600
PYDAGEYPAN MYLEGSDQHR GWFQSSLLVS TSIEERAPYK SILTHGFTVD
610 620 630 640 650
EKGEKMSKSK GNVVAPQEVS KKFGTEILRL WVATSDYSGD IKISDGILKQ
660 670 680 690 700
VAEQYRKIRN TIRFLLANVN DLEEIKLTNP SMIDRWILAR SKEVFDEVVA
710 720 730 740 750
LFGKYDFSKA FNILNNFIVT ELSAIYMDVC KDRLYCEPLN SEKRRNSQST
760 770 780 790 800
MAVIVKELIS LLAPVLTYTM DEAVEHAPKV IKEDAKDVFD FVYTPLTAVE
810 820 830 840 850
NPIDEEILEI RRRFFEIVDR LKKEKVIKDT LELAIETNYD KLLVDEMADF
860 870 880 890 900
FVVSLISDNI EGETLDEFHI SDEQFVVKIK RSPLHKCPRC WRYLAEEEGA
910
LCERCEKAIN G
Length:911
Mass (Da):104,913
Last modified:March 24, 2009 - v1
Checksum:i2061D723A2AA6983
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM92881.1.
RefSeqiWP_015901933.1. NC_012115.1.
YP_002607107.1. NC_012115.1.

Genome annotation databases

EnsemblBacteriaiACM92881; ACM92881; NAMH_0696.
GeneIDi7504838.
KEGGinam:NAMH_0696.
PATRICi22676107. VBINauPro131435_0667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM92881.1 .
RefSeqi WP_015901933.1. NC_012115.1.
YP_002607107.1. NC_012115.1.

3D structure databases

ProteinModelPortali B9L8Z9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 598659.NAMH_0696.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM92881 ; ACM92881 ; NAMH_0696 .
GeneIDi 7504838.
KEGGi nam:NAMH_0696.
PATRICi 22676107. VBINauPro131435_0667.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NPRO598659:GH7N-694-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
    Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
    PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Entry informationi

Entry nameiSYI_NAUPA
AccessioniPrimary (citable) accession number: B9L8Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3