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B9L8L4 (NAPA_NAUPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:NAMH_0556
OrganismNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH) [Complete proteome] [HAMAP]
Taxonomic identifier598659 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia

Protein attributes

Sequence length937 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 937906Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186367

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9L8L4 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: B9177C838CC34AF6

FASTA937106,622
        10         20         30         40         50         60 
MSMNRREFLK TTAAAAAASA VGISIPSEAK AAAENTQAGW QWDKAVCRFC GTGCGIMVAV 

        70         80         90        100        110        120 
KDDKIVAVKG DPESPVNRGI NCIKGYFNAK IMYGADRLTQ PLLRMNDKGE FDKNGKFRPV 

       130        140        150        160        170        180 
SWKRAFDEME KQFKKYYNEL GPTGVAVFGS GQYTIQEGYA AVKLVKAGWR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVVGFY QTFGIDEPAG CYDDIEITDT VFVWGANMAE MHPILWSRVS DAKLNNPDKY 

       250        260        270        280        290        300 
FIVNLSTYRN RCSNLADMEI IFRPNADLAI MNYLGREILK RNAVNWDFVK KHTIFATGHA 

       310        320        330        340        350        360 
DIGYGMRNPN SPHVTDKERD TVAKQVAKVV TEEEAVALSP IGKWKAGDEM QMTHTAGKKP 

       370        380        390        400        410        420 
FWHWQISFDD FKKAVEPYTL DYVAKVAKGD PDESLDSFKR KLQKLADLYC DPNRKALSFW 

       430        440        450        460        470        480 
TMGFNQHVRG SWINELVYMV HLLLGKQSQP GSGAFSLTGQ PSACGTAREV GTFAHRLPAD 

       490        500        510        520        530        540 
MLVGNPKHRK ITEKMWKIPE GTLNPKVGSH FLKIMRDIED GKIKWAWVHV NNPWQNTANA 

       550        560        570        580        590        600 
NHWIKAARTM DNFIVVNEAY PGVSARVADL ILPVSMIYEK WGAYGNAERR TQHWRQQVTP 

       610        620        630        640        650        660 
PGEAMPDLWT IMEFSKRFKL KEVWKAHPQA KLPSVLDKAQ AMGYDPEMTL FDVLFNRPEY 

       670        680        690        700        710        720 
RKNFPWPDPI AKNPQTGKLH ANTEAGGDGR TIIGADGKEW KGYGFFVHKA LWEEYRKFGL 

       730        740        750        760        770        780 
GHAHDLADFD TYHKVRGLRW PVVNGKETKW RFNAEYDPYA RKYAKPGEKF AFYGPLLKAL 

       790        800        810        820        830        840 
KRGDLKGPKT KQKYSLKNKA KIFWRPFMVH PEDPKYDTKG YNFWLCTGRV LEHWHSGTMT 

       850        860        870        880        890        900 
MRVPELYRAM PEALCYMNPK DAKELGLNRF DLVVVESRRG KVKARVETRG RNKPPRGLVY 

       910        920        930 
VPWFDEKVLI NKVCLDATCP LSKETDYKKA AVKVYKA 

« Hide

References

[1]"Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001279 Genomic DNA. Translation: ACM93598.1.
RefSeqYP_002606972.1. NC_012115.1.

3D structure databases

ProteinModelPortalB9L8L4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING598659.NAMH_0556.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM93598; ACM93598; NAMH_0556.
GeneID7504350.
KEGGnam:NAMH_0556.
PATRIC22675831. VBINauPro131435_0533.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycNPRO598659:GH7N-554-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_NAUPA
AccessionPrimary (citable) accession number: B9L8L4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families