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B9L8L4

- NAPA_NAUPA

UniProt

B9L8L4 - NAPA_NAUPA

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciNPRO598659:GH7N-554-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:NAMH_0556
    OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
    Taxonomic identifieri598659 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
    ProteomesiUP000000448: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 937906Periplasmic nitrate reductasePRO_1000186367Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi598659.NAMH_0556.

    Structurei

    3D structure databases

    ProteinModelPortaliB9L8L4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiFNQHQRG.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B9L8L4-1 [UniParc]FASTAAdd to Basket

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    MSMNRREFLK TTAAAAAASA VGISIPSEAK AAAENTQAGW QWDKAVCRFC    50
    GTGCGIMVAV KDDKIVAVKG DPESPVNRGI NCIKGYFNAK IMYGADRLTQ 100
    PLLRMNDKGE FDKNGKFRPV SWKRAFDEME KQFKKYYNEL GPTGVAVFGS 150
    GQYTIQEGYA AVKLVKAGWR SNNIDPNARH CMASAVVGFY QTFGIDEPAG 200
    CYDDIEITDT VFVWGANMAE MHPILWSRVS DAKLNNPDKY FIVNLSTYRN 250
    RCSNLADMEI IFRPNADLAI MNYLGREILK RNAVNWDFVK KHTIFATGHA 300
    DIGYGMRNPN SPHVTDKERD TVAKQVAKVV TEEEAVALSP IGKWKAGDEM 350
    QMTHTAGKKP FWHWQISFDD FKKAVEPYTL DYVAKVAKGD PDESLDSFKR 400
    KLQKLADLYC DPNRKALSFW TMGFNQHVRG SWINELVYMV HLLLGKQSQP 450
    GSGAFSLTGQ PSACGTAREV GTFAHRLPAD MLVGNPKHRK ITEKMWKIPE 500
    GTLNPKVGSH FLKIMRDIED GKIKWAWVHV NNPWQNTANA NHWIKAARTM 550
    DNFIVVNEAY PGVSARVADL ILPVSMIYEK WGAYGNAERR TQHWRQQVTP 600
    PGEAMPDLWT IMEFSKRFKL KEVWKAHPQA KLPSVLDKAQ AMGYDPEMTL 650
    FDVLFNRPEY RKNFPWPDPI AKNPQTGKLH ANTEAGGDGR TIIGADGKEW 700
    KGYGFFVHKA LWEEYRKFGL GHAHDLADFD TYHKVRGLRW PVVNGKETKW 750
    RFNAEYDPYA RKYAKPGEKF AFYGPLLKAL KRGDLKGPKT KQKYSLKNKA 800
    KIFWRPFMVH PEDPKYDTKG YNFWLCTGRV LEHWHSGTMT MRVPELYRAM 850
    PEALCYMNPK DAKELGLNRF DLVVVESRRG KVKARVETRG RNKPPRGLVY 900
    VPWFDEKVLI NKVCLDATCP LSKETDYKKA AVKVYKA 937
    Length:937
    Mass (Da):106,622
    Last modified:March 24, 2009 - v1
    Checksum:iB9177C838CC34AF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001279 Genomic DNA. Translation: ACM93598.1.
    RefSeqiYP_002606972.1. NC_012115.1.

    Genome annotation databases

    EnsemblBacteriaiACM93598; ACM93598; NAMH_0556.
    GeneIDi7504350.
    KEGGinam:NAMH_0556.
    PATRICi22675831. VBINauPro131435_0533.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001279 Genomic DNA. Translation: ACM93598.1 .
    RefSeqi YP_002606972.1. NC_012115.1.

    3D structure databases

    ProteinModelPortali B9L8L4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 598659.NAMH_0556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM93598 ; ACM93598 ; NAMH_0556 .
    GeneIDi 7504350.
    KEGGi nam:NAMH_0556.
    PATRICi 22675831. VBINauPro131435_0533.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi FNQHQRG.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci NPRO598659:GH7N-554-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
      Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
      PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1463 / DSM 18972 / AmH.

    Entry informationi

    Entry nameiNAPA_NAUPA
    AccessioniPrimary (citable) accession number: B9L8L4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3