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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei84Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei422Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei426Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei532Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei580Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei607Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei903Substrate; via amide nitrogenUniRule annotation1
Binding sitei911Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei928Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:NAMH_0556
OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Taxonomic identifieri598659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
Proteomesi

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_100018636732 – 937Periplasmic nitrate reductaseUniRule annotationAdd BLAST906

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiB9L8L4

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi598659.NAMH_0556

Structurei

3D structure databases

ProteinModelPortaliB9L8L4
SMRiB9L8L4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 964Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni827 – 836Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9L8L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMNRREFLK TTAAAAAASA VGISIPSEAK AAAENTQAGW QWDKAVCRFC
60 70 80 90 100
GTGCGIMVAV KDDKIVAVKG DPESPVNRGI NCIKGYFNAK IMYGADRLTQ
110 120 130 140 150
PLLRMNDKGE FDKNGKFRPV SWKRAFDEME KQFKKYYNEL GPTGVAVFGS
160 170 180 190 200
GQYTIQEGYA AVKLVKAGWR SNNIDPNARH CMASAVVGFY QTFGIDEPAG
210 220 230 240 250
CYDDIEITDT VFVWGANMAE MHPILWSRVS DAKLNNPDKY FIVNLSTYRN
260 270 280 290 300
RCSNLADMEI IFRPNADLAI MNYLGREILK RNAVNWDFVK KHTIFATGHA
310 320 330 340 350
DIGYGMRNPN SPHVTDKERD TVAKQVAKVV TEEEAVALSP IGKWKAGDEM
360 370 380 390 400
QMTHTAGKKP FWHWQISFDD FKKAVEPYTL DYVAKVAKGD PDESLDSFKR
410 420 430 440 450
KLQKLADLYC DPNRKALSFW TMGFNQHVRG SWINELVYMV HLLLGKQSQP
460 470 480 490 500
GSGAFSLTGQ PSACGTAREV GTFAHRLPAD MLVGNPKHRK ITEKMWKIPE
510 520 530 540 550
GTLNPKVGSH FLKIMRDIED GKIKWAWVHV NNPWQNTANA NHWIKAARTM
560 570 580 590 600
DNFIVVNEAY PGVSARVADL ILPVSMIYEK WGAYGNAERR TQHWRQQVTP
610 620 630 640 650
PGEAMPDLWT IMEFSKRFKL KEVWKAHPQA KLPSVLDKAQ AMGYDPEMTL
660 670 680 690 700
FDVLFNRPEY RKNFPWPDPI AKNPQTGKLH ANTEAGGDGR TIIGADGKEW
710 720 730 740 750
KGYGFFVHKA LWEEYRKFGL GHAHDLADFD TYHKVRGLRW PVVNGKETKW
760 770 780 790 800
RFNAEYDPYA RKYAKPGEKF AFYGPLLKAL KRGDLKGPKT KQKYSLKNKA
810 820 830 840 850
KIFWRPFMVH PEDPKYDTKG YNFWLCTGRV LEHWHSGTMT MRVPELYRAM
860 870 880 890 900
PEALCYMNPK DAKELGLNRF DLVVVESRRG KVKARVETRG RNKPPRGLVY
910 920 930
VPWFDEKVLI NKVCLDATCP LSKETDYKKA AVKVYKA
Length:937
Mass (Da):106,622
Last modified:March 24, 2009 - v1
Checksum:iB9177C838CC34AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001279 Genomic DNA Translation: ACM93598.1

Genome annotation databases

EnsemblBacteriaiACM93598; ACM93598; NAMH_0556
KEGGinam:NAMH_0556

Similar proteinsi

Entry informationi

Entry nameiNAPA_NAUPA
AccessioniPrimary (citable) accession number: B9L8L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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