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B9L8L4

- NAPA_NAUPA

UniProt

B9L8L4 - NAPA_NAUPA

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Protein

Periplasmic nitrate reductase

Gene
napA, NAMH_0556
Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciNPRO598659:GH7N-554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:NAMH_0556
OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Taxonomic identifieri598659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
ProteomesiUP000000448: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed predictionAdd
BLAST
Chaini32 – 937906Periplasmic nitrate reductaseUniRule annotationPRO_1000186367Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi598659.NAMH_0556.

Structurei

3D structure databases

ProteinModelPortaliB9L8L4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFNQHQRG.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9L8L4-1 [UniParc]FASTAAdd to Basket

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MSMNRREFLK TTAAAAAASA VGISIPSEAK AAAENTQAGW QWDKAVCRFC    50
GTGCGIMVAV KDDKIVAVKG DPESPVNRGI NCIKGYFNAK IMYGADRLTQ 100
PLLRMNDKGE FDKNGKFRPV SWKRAFDEME KQFKKYYNEL GPTGVAVFGS 150
GQYTIQEGYA AVKLVKAGWR SNNIDPNARH CMASAVVGFY QTFGIDEPAG 200
CYDDIEITDT VFVWGANMAE MHPILWSRVS DAKLNNPDKY FIVNLSTYRN 250
RCSNLADMEI IFRPNADLAI MNYLGREILK RNAVNWDFVK KHTIFATGHA 300
DIGYGMRNPN SPHVTDKERD TVAKQVAKVV TEEEAVALSP IGKWKAGDEM 350
QMTHTAGKKP FWHWQISFDD FKKAVEPYTL DYVAKVAKGD PDESLDSFKR 400
KLQKLADLYC DPNRKALSFW TMGFNQHVRG SWINELVYMV HLLLGKQSQP 450
GSGAFSLTGQ PSACGTAREV GTFAHRLPAD MLVGNPKHRK ITEKMWKIPE 500
GTLNPKVGSH FLKIMRDIED GKIKWAWVHV NNPWQNTANA NHWIKAARTM 550
DNFIVVNEAY PGVSARVADL ILPVSMIYEK WGAYGNAERR TQHWRQQVTP 600
PGEAMPDLWT IMEFSKRFKL KEVWKAHPQA KLPSVLDKAQ AMGYDPEMTL 650
FDVLFNRPEY RKNFPWPDPI AKNPQTGKLH ANTEAGGDGR TIIGADGKEW 700
KGYGFFVHKA LWEEYRKFGL GHAHDLADFD TYHKVRGLRW PVVNGKETKW 750
RFNAEYDPYA RKYAKPGEKF AFYGPLLKAL KRGDLKGPKT KQKYSLKNKA 800
KIFWRPFMVH PEDPKYDTKG YNFWLCTGRV LEHWHSGTMT MRVPELYRAM 850
PEALCYMNPK DAKELGLNRF DLVVVESRRG KVKARVETRG RNKPPRGLVY 900
VPWFDEKVLI NKVCLDATCP LSKETDYKKA AVKVYKA 937
Length:937
Mass (Da):106,622
Last modified:March 24, 2009 - v1
Checksum:iB9177C838CC34AF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM93598.1.
RefSeqiYP_002606972.1. NC_012115.1.

Genome annotation databases

EnsemblBacteriaiACM93598; ACM93598; NAMH_0556.
GeneIDi7504350.
KEGGinam:NAMH_0556.
PATRICi22675831. VBINauPro131435_0533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM93598.1 .
RefSeqi YP_002606972.1. NC_012115.1.

3D structure databases

ProteinModelPortali B9L8L4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 598659.NAMH_0556.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM93598 ; ACM93598 ; NAMH_0556 .
GeneIDi 7504350.
KEGGi nam:NAMH_0556.
PATRICi 22675831. VBINauPro131435_0533.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi FNQHQRG.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci NPRO598659:GH7N-554-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
    Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
    PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Entry informationi

Entry nameiNAPA_NAUPA
AccessioniPrimary (citable) accession number: B9L8L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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