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B9L8L4

- NAPA_NAUPA

UniProt

B9L8L4 - NAPA_NAUPA

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciNPRO598659:GH7N-554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:NAMH_0556
OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Taxonomic identifieri598659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
ProteomesiUP000000448: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 937906Periplasmic nitrate reductasePRO_1000186367Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi598659.NAMH_0556.

Structurei

3D structure databases

ProteinModelPortaliB9L8L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFNQHQRG.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9L8L4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMNRREFLK TTAAAAAASA VGISIPSEAK AAAENTQAGW QWDKAVCRFC
60 70 80 90 100
GTGCGIMVAV KDDKIVAVKG DPESPVNRGI NCIKGYFNAK IMYGADRLTQ
110 120 130 140 150
PLLRMNDKGE FDKNGKFRPV SWKRAFDEME KQFKKYYNEL GPTGVAVFGS
160 170 180 190 200
GQYTIQEGYA AVKLVKAGWR SNNIDPNARH CMASAVVGFY QTFGIDEPAG
210 220 230 240 250
CYDDIEITDT VFVWGANMAE MHPILWSRVS DAKLNNPDKY FIVNLSTYRN
260 270 280 290 300
RCSNLADMEI IFRPNADLAI MNYLGREILK RNAVNWDFVK KHTIFATGHA
310 320 330 340 350
DIGYGMRNPN SPHVTDKERD TVAKQVAKVV TEEEAVALSP IGKWKAGDEM
360 370 380 390 400
QMTHTAGKKP FWHWQISFDD FKKAVEPYTL DYVAKVAKGD PDESLDSFKR
410 420 430 440 450
KLQKLADLYC DPNRKALSFW TMGFNQHVRG SWINELVYMV HLLLGKQSQP
460 470 480 490 500
GSGAFSLTGQ PSACGTAREV GTFAHRLPAD MLVGNPKHRK ITEKMWKIPE
510 520 530 540 550
GTLNPKVGSH FLKIMRDIED GKIKWAWVHV NNPWQNTANA NHWIKAARTM
560 570 580 590 600
DNFIVVNEAY PGVSARVADL ILPVSMIYEK WGAYGNAERR TQHWRQQVTP
610 620 630 640 650
PGEAMPDLWT IMEFSKRFKL KEVWKAHPQA KLPSVLDKAQ AMGYDPEMTL
660 670 680 690 700
FDVLFNRPEY RKNFPWPDPI AKNPQTGKLH ANTEAGGDGR TIIGADGKEW
710 720 730 740 750
KGYGFFVHKA LWEEYRKFGL GHAHDLADFD TYHKVRGLRW PVVNGKETKW
760 770 780 790 800
RFNAEYDPYA RKYAKPGEKF AFYGPLLKAL KRGDLKGPKT KQKYSLKNKA
810 820 830 840 850
KIFWRPFMVH PEDPKYDTKG YNFWLCTGRV LEHWHSGTMT MRVPELYRAM
860 870 880 890 900
PEALCYMNPK DAKELGLNRF DLVVVESRRG KVKARVETRG RNKPPRGLVY
910 920 930
VPWFDEKVLI NKVCLDATCP LSKETDYKKA AVKVYKA
Length:937
Mass (Da):106,622
Last modified:March 24, 2009 - v1
Checksum:iB9177C838CC34AF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM93598.1.
RefSeqiYP_002606972.1. NC_012115.1.

Genome annotation databases

EnsemblBacteriaiACM93598; ACM93598; NAMH_0556.
GeneIDi7504350.
KEGGinam:NAMH_0556.
PATRICi22675831. VBINauPro131435_0533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM93598.1 .
RefSeqi YP_002606972.1. NC_012115.1.

3D structure databases

ProteinModelPortali B9L8L4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 598659.NAMH_0556.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM93598 ; ACM93598 ; NAMH_0556 .
GeneIDi 7504350.
KEGGi nam:NAMH_0556.
PATRICi 22675831. VBINauPro131435_0533.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi FNQHQRG.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci NPRO598659:GH7N-554-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
    Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
    PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Entry informationi

Entry nameiNAPA_NAUPA
AccessioniPrimary (citable) accession number: B9L8L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 29, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3