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B9L7E1 (BIOB_NAUPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:NAMH_0112
OrganismNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH) [Complete proteome] [HAMAP]
Taxonomic identifier598659 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276Biotin synthase HAMAP-Rule MF_01694
PRO_0000381492

Sites

Metal binding171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1531Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9L7E1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 9534343A069876FE

FASTA27630,734
        10         20         30         40         50         60 
MKKIYLCAIS NIRSGACNED CKFCTQSVKW GADINRYKQK DLKTIVNEAK LAKKNGATGF 

        70         80         90        100        110        120 
CLVTSGKGLD DKTLEYVCSA AKSVIKEVDI SIIACNGTAG KDSLKELKKA GVKIYNHNLE 

       130        140        150        160        170        180 
TSREYYPKIC STHTWDERFE TCENIKSVGL QLCCGGIFGM GESNEDIESF IRSLKELKPN 

       190        200        210        220        230        240 
GIPLNFFIEN EKLPLKATHN KDFALKTVKR FANEFKEAII MLAGGREIVF GNEWTEALKV 

       250        260        270 
GANSIVIGDY LTTKGERPDR DLEILLNEGF EIANEC 

« Hide

References

[1]"Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001279 Genomic DNA. Translation: ACM92846.1.
RefSeqYP_002606549.1. NC_012115.1.

3D structure databases

ProteinModelPortalB9L7E1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING598659.NAMH_0112.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM92846; ACM92846; NAMH_0112.
GeneID7503943.
KEGGnam:NAMH_0112.
PATRIC22674943. VBINauPro131435_0107.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK08508.

Enzyme and pathway databases

BioCycNPRO598659:GH7N-112-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NAUPA
AccessionPrimary (citable) accession number: B9L7E1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways