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B9L7E1

- BIOB_NAUPA

UniProt

B9L7E1 - BIOB_NAUPA

Protein

Biotin synthase

Gene

bioB

Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi24 – 241Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciNPRO598659:GH7N-112-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:NAMH_0112
    OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
    Taxonomic identifieri598659 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
    ProteomesiUP000000448: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276Biotin synthasePRO_0000381492Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi598659.NAMH_0112.

    Structurei

    3D structure databases

    ProteinModelPortaliB9L7E1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiFEIANEC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B9L7E1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIYLCAIS NIRSGACNED CKFCTQSVKW GADINRYKQK DLKTIVNEAK    50
    LAKKNGATGF CLVTSGKGLD DKTLEYVCSA AKSVIKEVDI SIIACNGTAG 100
    KDSLKELKKA GVKIYNHNLE TSREYYPKIC STHTWDERFE TCENIKSVGL 150
    QLCCGGIFGM GESNEDIESF IRSLKELKPN GIPLNFFIEN EKLPLKATHN 200
    KDFALKTVKR FANEFKEAII MLAGGREIVF GNEWTEALKV GANSIVIGDY 250
    LTTKGERPDR DLEILLNEGF EIANEC 276
    Length:276
    Mass (Da):30,734
    Last modified:March 24, 2009 - v1
    Checksum:i9534343A069876FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001279 Genomic DNA. Translation: ACM92846.1.
    RefSeqiYP_002606549.1. NC_012115.1.

    Genome annotation databases

    EnsemblBacteriaiACM92846; ACM92846; NAMH_0112.
    GeneIDi7503943.
    KEGGinam:NAMH_0112.
    PATRICi22674943. VBINauPro131435_0107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001279 Genomic DNA. Translation: ACM92846.1 .
    RefSeqi YP_002606549.1. NC_012115.1.

    3D structure databases

    ProteinModelPortali B9L7E1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 598659.NAMH_0112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM92846 ; ACM92846 ; NAMH_0112 .
    GeneIDi 7503943.
    KEGGi nam:NAMH_0112.
    PATRICi 22674943. VBINauPro131435_0107.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi FEIANEC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci NPRO598659:GH7N-112-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
      Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
      PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1463 / DSM 18972 / AmH.

    Entry informationi

    Entry nameiBIOB_NAUPA
    AccessioniPrimary (citable) accession number: B9L7E1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3