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B9L7E1

- BIOB_NAUPA

UniProt

B9L7E1 - BIOB_NAUPA

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Protein

Biotin synthase

Gene
bioB, NAMH_0112
Organism
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi24 – 241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNPRO598659:GH7N-112-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:NAMH_0112
OrganismiNautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Taxonomic identifieri598659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNautilialesNautiliaceaeNautilia
ProteomesiUP000000448: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Biotin synthaseUniRule annotationPRO_0000381492Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi598659.NAMH_0112.

Structurei

3D structure databases

ProteinModelPortaliB9L7E1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiFEIANEC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9L7E1-1 [UniParc]FASTAAdd to Basket

« Hide

MKKIYLCAIS NIRSGACNED CKFCTQSVKW GADINRYKQK DLKTIVNEAK    50
LAKKNGATGF CLVTSGKGLD DKTLEYVCSA AKSVIKEVDI SIIACNGTAG 100
KDSLKELKKA GVKIYNHNLE TSREYYPKIC STHTWDERFE TCENIKSVGL 150
QLCCGGIFGM GESNEDIESF IRSLKELKPN GIPLNFFIEN EKLPLKATHN 200
KDFALKTVKR FANEFKEAII MLAGGREIVF GNEWTEALKV GANSIVIGDY 250
LTTKGERPDR DLEILLNEGF EIANEC 276
Length:276
Mass (Da):30,734
Last modified:March 24, 2009 - v1
Checksum:i9534343A069876FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM92846.1.
RefSeqiYP_002606549.1. NC_012115.1.

Genome annotation databases

EnsemblBacteriaiACM92846; ACM92846; NAMH_0112.
GeneIDi7503943.
KEGGinam:NAMH_0112.
PATRICi22674943. VBINauPro131435_0107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001279 Genomic DNA. Translation: ACM92846.1 .
RefSeqi YP_002606549.1. NC_012115.1.

3D structure databases

ProteinModelPortali B9L7E1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 598659.NAMH_0112.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM92846 ; ACM92846 ; NAMH_0112 .
GeneIDi 7503943.
KEGGi nam:NAMH_0112.
PATRICi 22674943. VBINauPro131435_0107.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi FEIANEC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci NPRO598659:GH7N-112-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola."
    Campbell B.J., Smith J.L., Hanson T.E., Klotz M.G., Stein L.Y., Lee C.K., Wu D., Robinson J.M., Khouri H.M., Eisen J.A., Cary S.C.
    PLoS Genet. 5:E1000362-E1000362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1463 / DSM 18972 / AmH.

Entry informationi

Entry nameiBIOB_NAUPA
AccessioniPrimary (citable) accession number: B9L7E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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