B9L4S1 (B9L4S1_THERP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Pantothenate synthetase HAMAP MF_00158
Short name=PS HAMAP MF_00158
EC=6.3.2.1 HAMAP MF_00158

Alternative name(s):
Pantoate--beta-alanine ligase HAMAP MF_00158
Pantoate-activating enzyme HAMAP MF_00158

Gene names

Name:	panC HAMAP MF_00158 EMBL ACM07302.1
Ordered Locus Names:	trd_A0785

Encoded on

Plasmid Tros

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm By similarity HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family. HAMAP MF_00158

Ontologies

Keywords
Biological process	Pantothenate biosynthesis HAMAP MF_00158
Cellular component	Cytoplasm HAMAP MF_00158
Ligand	ATP-binding HAMAP MF_00158 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00158 EMBL ACM07302.1
Technical term	Complete proteome Plasmid EMBL ACM07302.1
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	28 – 35	8	ATP By similarity HAMAP MF_00158
	Nucleotide binding	146 – 149	4	ATP By similarity HAMAP MF_00158
	Nucleotide binding	183 – 186	4	ATP By similarity HAMAP MF_00158
Sites
	Active site	35	1	Proton donor By similarity HAMAP MF_00158
	Binding site	59	1	Beta-alanine By similarity HAMAP MF_00158
	Binding site	59	1	Pantoate By similarity HAMAP MF_00158
	Binding site	152	1	Pantoate By similarity HAMAP MF_00158
	Binding site	175	1	ATP; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00158

Sequences

	Sequence		Length	Mass (Da)
	B9L4S1 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 488131F6FF696C55	FASTA	282	31,533
	10 20 30 40 50 60 MHLFRTIAEL RIWRSQQASR SVGFVPTMGY LHEGHLALVR RARAENETVV VSIFVNPLQF 70 80 90 100 110 120 GPQEDYDRYP RDLDRDLYLL EREGVDAVFA PSVSEMYPSG FSTAVVVTGP IAERLEGEAR 130 140 150 160 170 180 PGHFRGVATV VTRLFGLVQP QRAYFGWKDA QQVLVVQRLV QDLALPVDIV PLPTVREADG 190 200 210 220 230 240 LAMSSRNVYL SPEERVAASA IPRALFAALE RFRSGERAAA AFRELVQRTL TTTPVRLEYV 250 260 270 280 SVSDLETFQE VEYVERPALL LVAAWIGTTR LIDNVLLDPG AP

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001276 Genomic DNA. Translation: ACM07302.1.
RefSeq	YP_002524067.1. NC_011961.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B9L4S1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7356597.
GenomeReviews	Gene locus trd_A0785 in contig CP001276_GR.
KEGG	tro:trd_A0785.
PATRIC	23916619. VBITheRos91376_2644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNMPIQI.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans-rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit. TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B9L4S1_THERP

Accession

Primary (citable) accession number: B9L4S1

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)