B9L2J1 (ACDH_THERP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetaldehyde dehydrogenase
EC=1.2.1.10

Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]

Gene names

Ordered Locus Names:

trd_1393

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Thermomicrobia › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium › Thermomicrobium roseum

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01657
Catalytic activity	Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP-Rule MF_01657
Sequence similarities	Belongs to the acetaldehyde dehydrogenase family.
Sequence caution	The sequence ACM04754.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: HAMAP acetaldehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Acetaldehyde dehydrogenase HAMAP-Rule MF_01657		PRO_0000387748
Regions
	Nucleotide binding	14 – 17	4	NAD By similarity
	Nucleotide binding	160 – 168	9	NAD By similarity
Sites
	Active site	129	1	Acyl-thioester intermediate By similarity
	Binding site	280	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9L2J1 [UniParc]. Last modified November 3, 2009. Version 2. Checksum: 640FEDC11586B542	FASTA	308	33,155
	10 20 30 40 50 60 MGERQAVKVA ILGTGNIGTD LMYKLLRNPG HMELAMFAGI DPQSEGIARA KKLGIPTSYE 70 80 90 100 110 120 GIKAVLDDPE IRIVFDATSA KAHVRHAKML REAGKIAIDL TPAARGPYVV PPVNLGAHLD 130 140 150 160 170 180 KDNVNLITCG GQATIPLVFA VSRVVPVRYA EIVSTVASKS AGPGTRQNID EFTFTTAHGL 190 200 210 220 230 240 EAIGGAEQGK AIIILNPAEP PIIMRNTVYV IPANEEFDQA AIVASVDQMV AEVQQYVPGY 250 260 270 280 290 300 RLKDAPVFDR RRTPWGERTI IAMLLEVEGA GDFLPPYSGN LDIMTAAAWR VGELFAQHLL GIRQEVAA

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27502 / DSM 5159 / P-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001275 Genomic DNA. Translation: ACM04754.1. Different initiation.
RefSeq	YP_002522598.1. NC_011959.1.
3D structure databases
ProteinModelPortal	B9L2J1.
ModBase	Search...
Protein-protein interaction databases
STRING	309801.trd_1393.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM04754; ACM04754; trd_1393.
GeneID	7354783.
KEGG	tro:trd_1393.
PATRIC	23913958. VBITheRos91376_1325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4569.
HOGENOM	HOG000052149.
KO	K04073.
ProtClustDB	PRK08300.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01657. Ac_ald_DH_ac.
InterPro	IPR003361. Acetaldehyde_dehydrogenase. IPR015426. Acetylaldehyde_DH_C. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. [Graphical view]
PANTHER	PTHR21123. PTHR21123. 1 hit.
Pfam	PF09290. AcetDehyd-dimer. 1 hit. PF01118. Semialdhyde_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015689. Actaldh_dh_actl. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACDH_THERP

Accession

Primary (citable) accession number: B9L2J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program