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B9L2E7 (B9L2E7_THERP) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172 EMBL ACM04918.1
Ordered Locus Names:trd_1349
OrganismThermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]
Taxonomic identifier309801 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6451Zinc By similarity HAMAP MF_00172
Metal binding6471Zinc By similarity HAMAP MF_00172
Metal binding7301Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
B9L2E7 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: B3A63ACCC411F050

FASTA76386,984
        10         20         30         40         50         60 
MAQSSVLGYP RIGKQRELKR ALERYWAGEA DAAALQETAR AVREAAWRVQ FQAGIDQIPC 

        70         80         90        100        110        120 
NDFSLYDHVL DTICLVGLVP SRFGSAEPEV SLPTYFAMAR GAPGVPALEL TKWFDTNYHY 

       130        140        150        160        170        180 
LVPEFDAPGP RLASAKPFLE FAEATSLLGR PAKPVLLGPI SLLLLGKYHQ RPLREHLEQL 

       190        200        210        220        230        240 
LPIYQEVLER LARAGAEWVQ MDEPVLVQDR SDEELQLLRA AYRVLGEASA RPKLMLQTYY 

       250        260        270        280        290        300 
GSLDDASWDV VMSLPIEGIG LDFVRDDGNR ALLNRHGFPQ GKVIGAGVVN GRNIWRTNLV 

       310        320        330        340        350        360 
EALEKLEEIA EHVEPERIWV QPSCTLLHLP HDVRLETKLP AAVRALLAFA EQRLEELVIL 

       370        380        390        400        410        420 
TRALNEGRAA ILEELEQNAE AFRLALDEVR VYRRDVRERL AALTEDDFRR QSPYEMREPL 

       430        440        450        460        470        480 
QRARFHLPLF PTTTIGSFPQ TPDVRQARAR WRAGKLSDED YWGFIRERIA EVIRRQEEIG 

       490        500        510        520        530        540 
LDVLVHGEYE RADMVEYFAE QLEGFVLTEH GWVQSYGSRV VRPPIIFGDV ARPRPMTVEV 

       550        560        570        580        590        600 
SRCAQSLTEK PVKGMLTGPV TILNWSFVRE DIPRREVAYQ IALALRDEVR DLEAAGISMI 

       610        620        630        640        650        660 
QIDEPALREG LPLRRADWPE YLEWAVRAFR LASSGVRDDT QIHSHMCYSE FNDIIEAIDA 

       670        680        690        700        710        720 
LDADVISIEN SRSRGELLQA FQEYRYPRQI GPGVYDVHSA RIPSVEEMLE LLERALAFLR 

       730        740        750        760 
PEQLWVNPDC GLKTRRPEEV WPSLSNMVEA ARRLRARFTA QGQ

« Hide

References

[1]"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum."
Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A.
PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001275 Genomic DNA. Translation: ACM04918.1.
RefSeqYP_002522554.1. NC_011959.1.

3D structure databases

ProteinModelPortalB9L2E7.
ModBaseSearch...

Protein-protein interaction databases

STRINGB9L2E7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7355762.
GenomeReviewsGene locus trd_1349 in contig CP001275_GR.
KEGGtro:trd_1349.
PATRIC23913872. VBITheRos91376_1282.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAGEHERND.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB9L2E7_THERP
AccessionPrimary (citable) accession number: B9L2E7
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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