B9L233 (B9L233_THERP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL ACM06114.1
Ordered Locus Names:	trd_1933

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 EMBL ACM06114.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	321	1	Nucleophile By similarity HAMAP MF_00685
	Active site	374	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B9L233 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 87A748D269871CAB	FASTA	647	74,676
	10 20 30 40 50 60 MTGRPVGPAC HDRSLLTEHD VYLFSEGTWL RAYEKMGAHP CVLDGEEGVY FAVWAPNAER 70 80 90 100 110 120 VSVVGDFNDW TPGKHPLCPR GSSGIWEGFI PGLGPGARYK YHLVSRYHGY QVFKADPYGF 130 140 150 160 170 180 RHECPPGTAS IVWDLTYEWQ DGDWMRRRGR ANALDAPMQI YEVHLGSWMR VPEEGNRWLT 190 200 210 220 230 240 YREIAPKLAE HVQRMGFTHV EFLPVMEHPF YGSWGYQITG YFAPTARYGT PQDFMALVDA 250 260 270 280 290 300 LHQQGIGVIL DWVPSHFPTD EHGLVFFDGT HLYEHADPRK GFHPDWHTAI FNYGRNEVRT 310 320 330 340 350 360 FLLSSAMFWL DRYHADGLRV DAVASMLYLD YSRGPGEWIP NEYGGRENLE AIAFLRRLNE 370 380 390 400 410 420 EIYRYYPDVQ TIAEESTAWP MVSRPTYLGG LGFGLKWDMG WMHDTLLYFS RDPIYRKYHH 430 440 450 460 470 480 HELTFRMLYA FTENFVLPLS HDEVVHGKGS LIAKMPGDDW QKFANLRLLY GYQYTQPGKK 490 500 510 520 530 540 LLFMGAELAQ WREWDHETSL DWHLLEYAPH RGVQRWLADL NRLACREPAL HERDVWPDGF 550 560 570 580 590 600 EWIDCNDAEN SVLVYLRKSC DPADTLLIVC NFTPVPRQNY RVGAPAPGFW RELLNSDARE 610 620 630 640 YGGSGWGNLG GVETVPVPWH GRPYSLVLTL PPLAILIFKR ATEGSVR

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001275 Genomic DNA. Translation: ACM06114.1.
RefSeq	YP_002523132.1. NC_011959.1.
3D structure databases
ProteinModelPortal	B9L233.
ModBase	Search...
Protein-protein interaction databases
STRING	B9L233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7354917.
GenomeReviews	Gene locus trd_1933 in contig CP001275_GR.
KEGG	tro:trd_1933.
PATRIC	23915028. VBITheRos91376_1855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DGTCLYE.
ProtClustDB	CLSK2808235.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B9L233_THERP

Accession

Primary (citable) accession number: B9L233

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)