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B9L1Z0 (B9L1Z0_THERP) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022
Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022
Gene names
Name:gltX HAMAP MF_00022 EMBL ACM04886.1
Ordered Locus Names:trd_1890
OrganismThermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]
Taxonomic identifier309801 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022

Subunit structure

Monomer By similarity. HAMAP MF_00022

Subcellular location

Cytoplasm By similarity HAMAP MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif12 – 2211"HIGH" region By similarity HAMAP MF_00022
Motif254 – 2585"KMSKS" region By similarity HAMAP MF_00022

Sites

Binding site2571ATP By similarity HAMAP MF_00022

Sequences

Sequence LengthMass (Da)Tools
B9L1Z0 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 28BBC47534336A0D

FASTA50657,642
        10         20         30         40         50         60 
MTTARVRVRF APSPTGDLHV GGARTALFNW LFARQHGGAF ILRIEDTDQA RLVGQSIAGI 

        70         80         90        100        110        120 
IEGLKWLGLE WDEGPDIGGP YGPYIQSQRL PLYREHARWL VEHGHAYYCF CTPERLERVR 

       130        140        150        160        170        180 
QEQRARGEPP GYDRHCRFLP PEEVEARLAR GEPAVIRFKM PLEGVTTIVD LLRGEITYEN 

       190        200        210        220        230        240 
RLLQDLVLLK SDGFPTYHLA NVVDDHFMEI SHILRAEEWI PSAPLHAQLY RAFGWEMPVL 

       250        260        270        280        290        300 
CHLPLILSPD GKGKLSKRHG ATGVLEFKRL GYLPEAMINY LALLGWAYDA EREIFSREEL 

       310        320        330        340        350        360 
IQLFRLEEVN PHPARFNFEK LLWMNQYYIN HVLSVDEFAR RCVPFLRDAG LVGPEADDPA 

       370        380        390        400        410        420 
SPAFAYVREV VALVKDRAKL LSEVPDLTRF FFADVEDYDP ELLLQRKVEP ERVLQALDRV 

       430        440        450        460        470        480 
IATLRQANFA DETDLEQRLR GLAEELGLKT GQLFMPIRVA VTGRTVSPGL FETLRVLGAE 

       490        500 
RSLERLARAR EKLAAYLAAR ASSATA

« Hide

References

[1]"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum."
Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A.
PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001275 Genomic DNA. Translation: ACM04886.1.
RefSeqYP_002523089.1. NC_011959.1.

3D structure databases

ProteinModelPortalB9L1Z0.
ModBaseSearch...

Protein-protein interaction databases

STRINGB9L1Z0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7354841.
GenomeReviewsGene locus trd_1890 in contig CP001275_GR.
KEGGtro:trd_1890.
PATRIC23914938. VBITheRos91376_1810.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMAHIPLI.
ProtClustDBPRK01406.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KOK01885.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMsTIGR00464. GltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9L1Z0_THERP
AccessionPrimary (citable) accession number: B9L1Z0
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info