B9L1L6 (DXS_THERP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	trd_1276

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 629	629	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000132945

Sequences

	Sequence		Length	Mass (Da)
	B9L1L6 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 9F16CC4C97BDEB45	FASTA	629	68,649
	10 20 30 40 50 60 MYLERIDSPQ DLKRLSVPEL EKLAEEIREA IIEVVLGKTG GHFAPNLGTV ELTLALHYVF 70 80 90 100 110 120 DSPRDKIVWD VGHQAYPHKL VTGRRDRFHT IRQEGGLSGF LQREESPHDH FGAGHASTSI 130 140 150 160 170 180 SAALGMAVAA KLRGDRYHTI AVIGDGALTG GMAYEALNHA GALQVPLIVV LNDNEMSIAP 190 200 210 220 230 240 NVGALARYLT RVRTDTRYRQ AKVEIERLLR RLPQGERLVE LSHRFLDGLK EVVYRTMIWE 250 260 270 280 290 300 ELGFTYIGPI DGHNLRELIE TFQLVKTFDS PVFVHVLTVK GKGYQPAEDD PFKHHSAAVK 310 320 330 340 350 360 VPGAPPTPPR YQDVFGQTLV ELAAKNDRIV AITAAMPDGT GLLPFAAAYP DRFFDVGIAE 370 380 390 400 410 420 QHAVTFAAGL ATQGLRPVCA IYSTFLQRAY DQVIHDVCIQ KLPVVFAMDR AGLVGEDGRT 430 440 450 460 470 480 HHGVFDVAYL RCLPNMVLMA PKDEDELRHM LATALAYEEG PIALRYPRGS GVGVPMLGEP 490 500 510 520 530 540 RVLPIGRAEL LREGSDVAIV ALGATVLPAE RAADILAERG IRATVINARF VKPLDRSLIL 550 560 570 580 590 600 DAARECGCLV TVEEAQLAGG FGSAVLETLA DAGLLIPVLR LGLADRFFDH ASQASLRRQA 610 620 GIDAESIASR TLAFLATHGR IETGAISDR

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27502 / DSM 5159 / P-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001275 Genomic DNA. Translation: ACM05181.1.
RefSeq	YP_002522483.1. NC_011959.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B9L1L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7355914.
GenomeReviews	Gene locus trd_1276 in contig CP001275_GR.
KEGG	tro:trd_1276.
PATRIC	23913726. VBITheRos91376_1211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EDGRTHH.
ProtClustDB	CLSK2807938.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_THERP

Accession

Primary (citable) accession number: B9L1L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program