B9L0U6 (DNAA_THERP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	trd_1166

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000189817
Regions
	Nucleotide binding	159 – 166	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B9L0U6 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 26D3F8861FF3FBEB	FASTA	465	52,221
	10 20 30 40 50 60 MRTKQLWQVA LGDLAKRVSR ANFETWLKHT ELVALEGERA TIAAPSSFAA EQLRNKFAAD 70 80 90 100 110 120 IQETLSLLLG RPIAVHFTVH GQDDEEHPVQ RRPQRRALAS EEGSASKQLS LTPSPEHGLN 130 140 150 160 170 180 PRYTFEKFVV GPNNRLAHAA ALAVADRPGE KFNPLFIYGG VGLGKTHLLH AIGHRALANR 190 200 210 220 230 240 PTLKVCYVSS EVFTNELINA IRHQRTDDFR NRYRTIDILM IDDIQFIAGK ESTQEEFFHT 250 260 270 280 290 300 FNALYQSGKQ IVISSDRPPR LIPDLADRLR SRFEGGLLAD IQPPDLETRQ AILIEKGREL 310 320 330 340 350 360 GVQMPSDVVE FVARRIESNI RELEGALNRI VALAQLTHQP ITLALAVEAL REGDARAVRE 370 380 390 400 410 420 QLTPELVLHA VCQHFRVSLA ELVGPGRRRE IVLPRQIAMY LLREELGLSL VEIGQRLGGR 430 440 450 460 DHTTVLHGVD KVEEQLRSDE QLRADVLAVR ARLYEDAKAP LASRH

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27502 / DSM 5159 / P-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001275 Genomic DNA. Translation: ACM05694.1.
RefSeq	YP_002522376.1. NC_011959.1.
3D structure databases
ProteinModelPortal	B9L0U6.
ModBase	Search...
Protein-protein interaction databases
STRING	B9L0U6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7354592.
GenomeReviews	Gene locus trd_1166 in contig CP001275_GR.
KEGG	tro:trd_1166.
PATRIC	23913506. VBITheRos91376_1103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CHTLNAL.
ProtClustDB	CLSK2807870.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_THERP

Accession

Primary (citable) accession number: B9L0U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program