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B9KZS7 (MDH_THERP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:trd_0921
OrganismThermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]
Taxonomic identifier309801 [NCBI]
Taxonomic lineageBacteriaChloroflexiThermomicrobialesThermomicrobiaceaeThermomicrobium

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Malate dehydrogenase HAMAP-Rule MF_00487
PRO_1000206447

Regions

Nucleotide binding9 – 146NAD By similarity
Nucleotide binding118 – 1203NAD By similarity

Sites

Active site1751Proton acceptor By similarity
Binding site331NAD By similarity
Binding site821Substrate By similarity
Binding site881Substrate By similarity
Binding site951NAD By similarity
Binding site1201Substrate By similarity
Binding site1511Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B9KZS7 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 0A78AEFCF128D9C5

FASTA30932,492
        10         20         30         40         50         60 
MRRKVSIIGA GAVGATTAQY LAARNIADLV LVDIVENLPQ GKALDLLEAG PVLGYDCQIV 

        70         80         90        100        110        120 
GSNSYDATAG SDVIVITSGS PRKPGMSRDD LLRVNMNIVR SVTEQAAPLS PDAVIIVVTN 

       130        140        150        160        170        180 
PLDAMCHVAL EASGFPPARV VGQAGVLDAA RFRAFVALEL GVSPRDVHAM VLGGHGDTMV 

       190        200        210        220        230        240 
PLPRYTTVSG IPITQLIPAD RIQAIVERTR DGGGEIVRLL GTSAFYAPAA SVAEMVEAVL 

       250        260        270        280        290        300 
LDANRVLAAS TYLTGQYGIH DLYVGVPIRL GAGGVKEIIE VELTDEERAA LHRSANAVRE 


LVQAMKQLT 

« Hide

References

[1]"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum."
Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A.
PLoS ONE 4:E4207-E4207(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27502 / DSM 5159 / P-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001275 Genomic DNA. Translation: ACM05183.1.
RefSeqYP_002522141.1. NC_011959.1.

3D structure databases

ProteinModelPortalB9KZS7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309801.trd_0921.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM05183; ACM05183; trd_0921.
GeneID7355233.
KEGGtro:trd_0921.
PATRIC23913020. VBITheRos91376_0871.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMALDLMEAQ.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycTROS309801:GI0S-913-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_THERP
AccessionPrimary (citable) accession number: B9KZS7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families