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B9KZ15 (B9KZ15_THERP) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036 EMBL ACM04869.1
Ordered Locus Names:trd_0726
OrganismThermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]
Taxonomic identifier309801 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5611Zinc By similarity HAMAP MF_00036
Metal binding5651Zinc By similarity HAMAP MF_00036
Metal binding6631Zinc By similarity HAMAP MF_00036
Metal binding6671Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
B9KZ15 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 390C585686C9D9AA

FASTA87898,118
        10         20         30         40         50         60 
MQSSEIRRAF IEFFAERGHV QVPSSSLIPS DPTVLLTTAG MQQMVPYFLG LERPPHTRLT 

        70         80         90        100        110        120 
SIQKCFRTVD IDEVGDESHL TFFEMLGNFS IGDYFKAEAI SWAWEFLTKW LGVPAERWYP 

       130        140        150        160        170        180 
TVHPDDEFSY QYWRDQIGVP TERIFKLEDN WWGPVGATGP NGPDSEIHYD RGMEYGCGRT 

       190        200        210        220        230        240 
ECGPGCDCGR FLETWNLVFM EFYKEADGTQ RPLPRKNIDT GMGLERISLI MQGVGSVFET 

       250        260        270        280        290        300 
DLFYPILSEA ATIAGVRYKE QARVDRSLRV IADHARGVTF LVGDGVFPSN EGRGYVLRRV 

       310        320        330        340        350        360 
LRRAVRHGKL LGIERPFLNQ LVDVVIELFS SYYPNLNEQR ERIHRVIRHE EEHFQRTLSA 

       370        380        390        400        410        420 
GLSRFEALLD QLQRSGEQVL PGDEAFRLYD TYGFPLELTE ELARESGFTV DRAGFERALE 

       430        440        450        460        470        480 
RQRELSRASV GRFADTQRQR AELYAQFSER PTEFVGYDRL ETTATIVGIL GLSDLRQEAE 

       490        500        510        520        530        540 
AGEEIELILD RTPFYGEAGG QVGDTGEIVA ETGIVTVEDT QRPTPELIVH RGRVREGSIR 

       550        560        570        580        590        600 
VGQSVRAIVD AERRAAIRRN HTATHLLHAA LRRVLGEHAV QAGSLVAPDR LRFDFSHHES 

       610        620        630        640        650        660 
VSPEQLRRIE EMVNEQIVRD LRVEVRYLPL RDALAEGAIA LFGEKYGDTV RVVSIDGFSK 

       670        680        690        700        710        720 
ELCGGTHVSH TGEIGAFLIT DETSVASGIR RIEAVTGLAA ARLARTLIDV SEEAARRLHV 

       730        740        750        760        770        780 
VPEQLPEAIE RLQQQVRDQQ REIERLAADL AAARLAPLVE RAARIDGFRV VSARIEVPSL 

       790        800        810        820        830        840 
DVLRRVGDRL RDAIGSGVVI LGTAIENRPM ILAMATPDAV QRGVHAGQVV QAVTPLLGGR 

       850        860        870 
GGGRPDIAQG GGADVSRLDE ALAAARGEVE RQLRNSTS

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References

[1]"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum."
Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A.
PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001275 Genomic DNA. Translation: ACM04869.1.
RefSeqYP_002521957.1. NC_011959.1.

3D structure databases

ProteinModelPortalB9KZ15.
ModBaseSearch...

Protein-protein interaction databases

STRINGB9KZ15.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7355504.
GenomeReviewsGene locus trd_0726 in contig CP001275_GR.
KEGGtro:trd_0726.
PATRIC23912650. VBITheRos91376_0696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAGESKTDQ.
ProtClustDBCLSK2807654.

Family and domain databases

HAMAPMF_00036_B. Ala_tRNA_synth_B.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_synth_euk/bac.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9KZ15_THERP
AccessionPrimary (citable) accession number: B9KZ15
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info