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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi498 – 4981MagnesiumUniRule annotation
Metal bindingi504 – 5041MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciTROS309801:GI0S-618-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:trd_0623
OrganismiThermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2)
Taxonomic identifieri309801 [NCBI]
Taxonomic lineageiBacteriaChloroflexiThermomicrobialesThermomicrobiaceaeThermomicrobium
ProteomesiUP000000447 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790Polyribonucleotide nucleotidyltransferasePRO_0000381924Add
BLAST

Proteomic databases

PRIDEiB9KYR9.

Interactioni

Protein-protein interaction databases

STRINGi309801.trd_0623.

Structurei

3D structure databases

ProteinModelPortaliB9KYR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini565 – 62460KHUniRule annotationAdd
BLAST
Domaini634 – 70269S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9KYR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPIIHERSI EIAGRLLTIE TGRVAEQADG AVLVRYGETV VLTTVVGAKQ
60 70 80 90 100
PVEGIDFFPL TVEYEEKMYA AGKIPGGFFR REGKPSEAAI LAARLTDRPI
110 120 130 140 150
RPLFPKGYRN EVQVISTVLS ADQENEPDVL SIIGASAALT LSDIPWYGPV
160 170 180 190 200
GAVRIGELDG ELVINPTSHQ LLESRMDIVV AGTADAILMV EGQANEISED
210 220 230 240 250
RFIEAVVRAH QEIKRIVAVQ LELQAVAGKP KREFVPPQEN VELKQQIADY
260 270 280 290 300
LGDRLREAVF NPDKTLRVQA TAALREEVIA HFVPNEPLAI GTPQSGLPTA
310 320 330 340 350
KEVGDLFDSL VKELVRRTIL EQGERPDGRK PDEIREIWIQ VGLLPRPHGS
360 370 380 390 400
ALFTRGQTQV LTVCTLGTKE EEQFLDSLGI EETKRYMHHY NFPPFSTGEI
410 420 430 440 450
RRLRGPSRRD IGHGALAERA LLAVLPSEDE FPYTMRLVSE VLSSNGSTSM
460 470 480 490 500
ASVCGSSLAL MDAGVPIRKP VAGVAMGLVT DQTTGRYTIL TDIQGIEDAL
510 520 530 540 550
GDMDFKVAGT RDGITAIQMD IKVMGITPEI MRDALEQARR GRLFILDKMS
560 570 580 590 600
EVIDAPRPEM SPYAPRILRI KIKPEQIGEV IGPGGRVIRA IQEQTGTKIS
610 620 630 640 650
IEEDGTVFIS AANEDAARRA VREIERLTRV PEVGEIFYGR VVTIIPSGAF
660 670 680 690 700
VEILPGKDGF LHISEIAPER VRSVEDVLKV GQEINVMVIG VRPDGKINLS
710 720 730 740 750
RKALLEKEAA ERAATAQAPA DGRSHQPRAP QRPSGTAQPE RRPGPPTPRR
760 770 780 790
PEQRGPSRPP RPQAQRSTPP PGQYRIGDRL KELLGEDEPN
Length:790
Mass (Da):86,688
Last modified:March 23, 2009 - v1
Checksum:i6295D2A0E06D767B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001275 Genomic DNA. Translation: ACM05386.1.
RefSeqiWP_012642019.1. NC_011959.1.
YP_002521861.1. NC_011959.1.

Genome annotation databases

EnsemblBacteriaiACM05386; ACM05386; trd_0623.
KEGGitro:trd_0623.
PATRICi23912454. VBITheRos91376_0604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001275 Genomic DNA. Translation: ACM05386.1.
RefSeqiWP_012642019.1. NC_011959.1.
YP_002521861.1. NC_011959.1.

3D structure databases

ProteinModelPortaliB9KYR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309801.trd_0623.

Proteomic databases

PRIDEiB9KYR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM05386; ACM05386; trd_0623.
KEGGitro:trd_0623.
PATRICi23912454. VBITheRos91376_0604.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciTROS309801:GI0S-618-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum."
    Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A.
    PLoS ONE 4:E4207-E4207(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27502 / DSM 5159 / P-2.

Entry informationi

Entry nameiPNP_THERP
AccessioniPrimary (citable) accession number: B9KYR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2009
Last sequence update: March 23, 2009
Last modified: March 31, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.