B9KY71 (DAPA_THERP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	trd_0414

Organism

Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) [Complete proteome] [HAMAP]

Taxonomic identifier

309801 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Thermomicrobiales › Thermomicrobiaceae › Thermomicrobium

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000134885
Regions
	Region	47 – 48	2	Pyruvate binding By similarity
Sites
	Active site	160	1	Schiff-base intermediate with substrate By similarity
	Binding site	105	1	Pyruvate By similarity
	Site	132	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9KY71 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 56C3753BC8634D0E	FASTA	302	31,674
	10 20 30 40 50 60 MLRGTFVALI TPFAGEEIDE PRLRDLVDWL IANRVDGLVP CGTTGETPSL SDTEWQRVAA 70 80 90 100 110 120 VVIEQAAGRV PVIVGTGTNS TMVTIQRTRV ARELGATAAM VVTPYYNKPQ QDGLYRHVAA 130 140 150 160 170 180 IADAVDLPLV IYNVPSRTGV NLAPETARRL LDIAPVIAFK DSSGSLDQVS ELVLAVGDRS 190 200 210 220 230 240 SVLSGDDSLT LPIIAVGGQG VVSVLANIAP AATATMVRAA LDGDLARARQ LHGELFPLAR 250 260 270 280 290 300 ALFIETNPVP VKTAAELLGL CSATVRLPLA PLAPANRERL LAALASCPHT ASLLARPMGE AA

References

[1]

"Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum." Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E., Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R., Ward N.L., Eisen J.A. PLoS ONE 4:E4207-E4207(2009) [PubMed: 19148287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27502 / DSM 5159 / P-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001275 Genomic DNA. Translation: ACM05517.1.
RefSeq	YP_002521663.1. NC_011959.1.
3D structure databases
ProteinModelPortal	B9KY71.
ModBase	Search...
Protein-protein interaction databases
STRING	B9KY71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7355460.
GenomeReviews	Gene locus trd_0414 in contig CP001275_GR.
KEGG	tro:trd_0414.
PATRIC	23912048. VBITheRos91376_0408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CEMEDSN.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_THERP

Accession

Primary (citable) accession number: B9KY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program