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B9KSE2

- LIPA_RHOSK

UniProt

B9KSE2 - LIPA_RHOSK

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Protein
Lipoyl synthase
Gene
lipA, RSKD131_1089
Organism
Rhodobacter sphaeroides (strain KD131 / KCTC 12085)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRSPH557760:GH1P-1099-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:RSKD131_1089
OrganismiRhodobacter sphaeroides (strain KD131 / KCTC 12085)
Taxonomic identifieri557760 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000001597: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Lipoyl synthaseUniRule annotation
PRO_1000124643Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi557760.RSKD131_1089.

Structurei

3D structure databases

ProteinModelPortaliB9KSE2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiTIRAVRH.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9KSE2-1 [UniParc]FASTAAdd to Basket

« Hide

MIGKLVRDLK IPDQRHPEKA HRPDNVQPKK PSWIRVKAPT SEGYKETRDI    50
IRGQKLATVC EEAGCPNVGE CWSQGHATMM IMGEICTRGC SFCNVATGRP 100
QALDAFEPGR VAHAVSQLGL KHVVVTSVDR DDLEDGGAEH FAQTIRAIRH 150
RAPATTIEVL VPDFLKCGPS ALETVVAARP DVFNHNLETV PGLYPEVRPG 200
ARYFHSLRLL QRAKELDPSI FTKSGIMVGL GEDRQGVLQV MDDMRSAEVD 250
FLTIGQYLQP TPKHHRVDRF VTPEEFAGYE KAAYGKGFLM VSATPLTRSS 300
YHAGDDFARL RDARQKRLGA 320
Length:320
Mass (Da):35,492
Last modified:March 24, 2009 - v1
Checksum:i0BF64485962BE698
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001150 Genomic DNA. Translation: ACM00949.1.
RefSeqiYP_002525450.1. NC_011963.1.

Genome annotation databases

EnsemblBacteriaiACM00949; ACM00949; RSKD131_1089.
GeneIDi7360504.
KEGGirsk:RSKD131_1089.
PATRICi23181277. VBIRhoSph125910_2502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001150 Genomic DNA. Translation: ACM00949.1 .
RefSeqi YP_002525450.1. NC_011963.1.

3D structure databases

ProteinModelPortali B9KSE2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 557760.RSKD131_1089.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM00949 ; ACM00949 ; RSKD131_1089 .
GeneIDi 7360504.
KEGGi rsk:RSKD131_1089.
PATRICi 23181277. VBIRhoSph125910_2502.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi TIRAVRH.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RSPH557760:GH1P-1099-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rhodobacter sphaeroides KD131."
    Lim S.-K., Kim S.J., Cha S.H., Oh Y.-K., Rhee H.-J., Kim M.-S., Lee J.K.
    J. Bacteriol. 191:1118-1119(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KD131 / KCTC 12085.

Entry informationi

Entry nameiLIPA_RHOSK
AccessioniPrimary (citable) accession number: B9KSE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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