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Protein

Argininosuccinate synthase

Gene

argG

Organism
Rhodobacter sphaeroides (strain KD131 / KCTC 12085)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH), Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei90CitrullineUniRule annotation1
Binding sitei95CitrullineUniRule annotation1
Binding sitei120ATP; via amide nitrogenUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei126AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei127AspartateUniRule annotation1
Binding sitei130CitrullineUniRule annotation1
Binding sitei181CitrullineUniRule annotation1
Binding sitei190CitrullineUniRule annotation1
Binding sitei266CitrullineUniRule annotation1
Binding sitei278CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:RSKD131_2610
OrganismiRhodobacter sphaeroides (strain KD131 / KCTC 12085)
Taxonomic identifieri557760 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000001597 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001162901 – 408Argininosuccinate synthaseAdd BLAST408

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB9KPT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9KPT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPKKVVLA YSGGLDTSII LKWLQTEYGC EVVTFTADLG QGEELEPARE
60 70 80 90 100
KAVMLGIKPE NIFIEDVREE FVRDFVFPMF RANALYEGLY LLGTSIARPL
110 120 130 140 150
IAKRLVEIAA QTGADAVAHG ATGKGNDQVR FELTAYALDP AIKVIAPWRE
160 170 180 190 200
WDLTSRTKLL EFAEQNQIPI AKNKRGEAPF SVDANLLHTS SEGRVLENPG
210 220 230 240 250
EEAPDYVYQR TVDPEKAPDA PEFVEIAFEK GDAVAINGEA MSPATILTKL
260 270 280 290 300
NELGGKHGVG RLDLVENRFV GMKSRGIYET PGGTILLEAH RGIEQITLDS
310 320 330 340 350
GAGHLKDSIM PRYAELIYNG FWYSPEREML QALIDKSQEH VTGTVRVKLY
360 370 380 390 400
KGFARTVARW SEHSLYSEKH VTFEEDAGAY DQKDAAGFIR LNALRLKLIA

TRNARVKG
Length:408
Mass (Da):45,244
Last modified:March 24, 2009 - v1
Checksum:iAE24E105324E0927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001150 Genomic DNA. Translation: ACM02470.1.
RefSeqiWP_002721701.1. NC_011963.1.

Genome annotation databases

EnsemblBacteriaiACM02470; ACM02470; RSKD131_2610.
KEGGirsk:RSKD131_2610.
PATRICi23184352. VBIRhoSph125910_4016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001150 Genomic DNA. Translation: ACM02470.1.
RefSeqiWP_002721701.1. NC_011963.1.

3D structure databases

ProteinModelPortaliB9KPT5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM02470; ACM02470; RSKD131_2610.
KEGGirsk:RSKD131_2610.
PATRICi23184352. VBIRhoSph125910_4016.

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_RHOSK
AccessioniPrimary (citable) accession number: B9KPT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.